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Effects of acute microinjections of thyroid hormone to the median preoptic nucleus of hypothyroid adult male rats on sleep, motor activity, and body temperature
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Effects of acute microinjections of thyroid hormone to the median preoptic nucleus of hypothyroid adult male rats on sleep, motor activity, and body temperature
Identifier
ETD_3107
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10005600001.ETD.000057457
Language
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(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Biology
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Hypothyroidism--Research
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Thyroid hormones--Metabolism
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Thyroid hormones--Physiological effect
Subject
(ID = SBJ-5);
(authority = ETD-LCSH)
Topic
Sleep disorders--Pathophysiology
Subject
(ID = SBJ-6);
(authority = ETD-LCSH)
Topic
Rats as laboratory animals
Abstract
(type = abstract)
Thyroid hormone's role as a genomic regulator of basal metabolic rate does not explain neurological changes in patients with dysthyroidism. Hypothyroidism, characterized by depressive symptoms including somnolence, intellectual deterioration, and coma, and hyperthyroidism, illustrated by symptoms including insomnia, anxiety, and seizures, imply a mechanism of thyroid hormone action which influences behavior. In adults, thyroid hormone is taken up to discrete brain areas by organic anion and monocarboxylate transport proteins. One site of distribution, the median preoptic nucleus (MePO), contains clusters of sleep-active neurons and is considered a key sleep-regulatory structure. To test the acute effect of thyroid hormone at this locus, we stereotaxically implanted adult hypothyroid rats with microinjection guide cannulae at the MePO and installed electrodes and miniature transmitters for recording EEG, EMG, core body temperature, and locomotor activity. The rats were rendered hypothyroid by administration of 0.02% 6-n-propyl-2-thiouracil in drinking water until body weight plateaued, indicating a hypothyroid condition. Rats were given a series of dosages (0.3 μg, 1 μg, 3 μg, or 10 μg in 0.25μL vehicle) of L-3,3´,5-triiodothyronine (T3) in four weekly 48-hour tests. Each week, rats were bilaterally microinjected with a control solution. After 24 hours of recording, each rat was microinjected with one of the above doses of T3 and recorded for an additional 24 hours. In seven rats with histologically-confirmed cannulae placements in the MePO, we observed significant effects on REM, non-REM, total sleep, core body temperature and motor activity analyzed by 2-way ANOVAs. For each sleep parameter the effect of dose of hormone was significant (p < 0.0001), as was the effect of time after injection (p<0.0001), except for in REM sleep. Temperature and activity also showed significant effects of dose (p < 0.02) and time (p < 0.001). There was no significant interaction between dose and time factors in any ANOVA. Since the effects of T3 were seen within hours of acute injection, the data are most consistent with a non-genomic mechanism of action of thyroid hormones in the adult brain. The mechanism of thyroid hormone action may be due to inhibition of the GABAA receptor, by an unknown protein phosphorylation effect, or by its actions as an adrenergic system analogue.
PhysicalDescription
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electronic resource
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vii, 41 p. : ill.
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application/pdf
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text/xml
Note
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M.S.
Note
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Includes bibliographical references
Note
(type = statement of responsibility)
by Steven Moffett
Name
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Moffett
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Steven
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1984-
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author
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Steven Moffett
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Morgan
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Mark D
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chair
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Advisory Committee
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Mark D Morgan
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NamePart
(type = family)
Martin
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(type = given)
Joseph V
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internal member
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Advisory Committee
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Joseph V Martin
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Saidel
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William M
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internal member
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Advisory Committee
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William M Saidel
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McIlroy
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Patrick J
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internal member
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Advisory Committee
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Patrick J McIlroy
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Camden Graduate School
Role
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school
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Camden Graduate School
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school
OriginInfo
DateCreated
(qualifier = exact)
2011
DateOther
(qualifier = exact);
(type = degree)
2011-01
Place
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(type = code)
xx
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
RelatedItem
(type = host)
TitleInfo
Title
Camden Graduate School Electronic Theses and Dissertations
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rucore10005600001
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NjNbRU
Identifier
(type = doi)
doi:10.7282/T3T72H38
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
(ID = PRH-1);
(type = personal)
Name
FamilyName
Moffett
GivenName
Steven
Role
Copyright Holder
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(ID = RE-1);
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Type
Permission or license
DateTime
2011-01-03 14:57:03
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Copyright holder
Name
Steven Moffett
Affiliation
Rutgers University. Camden Graduate School
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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ETD
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application/pdf
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application/x-tar
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1843200
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