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Identifications of polyphenols and quantification of anthocyanidins in grapes and grape-derived products
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Identifications of polyphenols and quantification of anthocyanidins in grapes and grape-derived products
Identifier
ETD_3070
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057721
Language
LanguageTerm
(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Medicinal Chemistry
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Polyphenols
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Grapes--Therapeutic use
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Phenolic acids
Subject
(ID = SBJ-5);
(authority = ETD-LCSH)
Topic
Anthocyanins
Abstract
(type = abstract)
Polyphenols in grapes and grape-derived products have attracted public and scientific attention due to their numerous protective roles to human health. A rapid and comprehensive qualitative method was developed to characterize the different classes of polyphenols, such as anthocyanins, flavonols, phenolic acids and flavanols/proanthocyanidins, in grapes and grape-derived products. The detection was achieved by two runs with same HPLC gradient in different MS ionization modes and mobile phase modifiers (positive mode and 0.4% trifluoroacetic acid for anthocyanins and flavonols, negative mode and 0.1% formic acid for phenolic acids and flavanols). Under the optimized LC/MS conditions and based on the analysis of the MS and UV data and in comparison with the authenticated standards, a total of 53 polyphenolic compounds were successfully separated and individually identified including 33 anthocyanins, 12 flavonols, 4 phenolic acids and 4 flavanols/proanthocyanidins. With the method developed, a survey was conducted to qualitatively assess and compare the composition of polyphenols among 29 grapes and grape-derived products. To facilitate the quantitation of the major class of polyphenolic anthocyanidins, a simple and precise acid assisted hydrolysis method was established for the quantitation of anthocyanidins in grape juice samples, grape berries and grape skins using LC/MS. Five most common anthocyanidins of delphenidin, petunidin, cyanidin, malvidin, and peonidin in the hydrolyzed grape extracts were included in the quantification study. The validation of this method showed that the recovery percentages of five anthocyanidins ranged from 98.59 % to 103.20% with the relative standard deviation (RSD) less than 5.03%. The qualitative method provided complete insight into the composition of polyphenols in grapes, and other grape-derived products. This quantitative method provides a rapid and accurate tool to quantitatively study individual anthocyanidin in grapes or grape juice samples for quality control and to facilitate the evaluation and comparison of new commercial grapes or grape juice products in market.
PhysicalDescription
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electronic resource
Extent
xii, 112 p. : ill.
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text/xml
Note
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M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Yanping Xu
Name
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(type = personal)
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Xu
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Yanping
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1980-
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author
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Yanping Xu
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Simon
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James E
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chair
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Advisory Committee
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James E Simon
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NamePart
(type = family)
Wu
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(type = given)
Qingli
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internal member
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Advisory Committee
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Qingli Wu
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LaVoie
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(type = given)
Edmond J
Role
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internal member
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Advisory Committee
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Edmond J LaVoie
Name
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NamePart
Rutgers University
Role
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degree grantor
Name
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Graduate School - New Brunswick
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school
OriginInfo
DateCreated
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2011
DateOther
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(type = degree)
2011-01
Place
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(type = code)
xx
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3RN37GS
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
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(ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
(ID = PRH-1);
(type = personal)
Name
FamilyName
Xu
GivenName
Yanping
Role
Copyright Holder
RightsEvent
(ID = RE-1);
(AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-12-21 16:57:01
AssociatedEntity
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(AUTHORITY = rulib)
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Copyright holder
Name
Yanping Xu
Affiliation
Rutgers University. Graduate School - New Brunswick
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License
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
(ID = RE-2);
(AUTHORITY = rulib)
Type
Embargo
DateTime
2011-01-31
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after August 2nd, 2011.
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Technical
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ETD
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application/pdf
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application/x-tar
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2078720
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