RUcore: Rutgers University Community Repository
MIR-9 targets OC2 in proliferating and differentiating neural stem cells
Descriptive
TitleInfo
Title
MIR-9 targets OC2 in proliferating and differentiating neural stem cells
Name
(type = personal)
NamePart
(type = family)
Davila
NamePart
(type = given)
Jorge
NamePart
(type = date)
1977-
DisplayForm
Jorge Davila
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Hart
NamePart
(type = given)
Ronald P
DisplayForm
Ronald P Hart
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Brewer
NamePart
(type = given)
Gary
DisplayForm
Gary Brewer
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Grumet
NamePart
(type = given)
Martin
DisplayForm
Martin Grumet
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Cai
NamePart
(type = given)
Li
DisplayForm
Li Cai
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2011
DateOther
(qualifier = exact);
(type = degree)
2011-05
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Subject
(authority = RUETD)
Topic
Cell and Developmental Biology
Subject
(authority = ETD-LCSH)
Topic
RNA
Subject
(authority = ETD-LCSH)
Topic
Neural stem cells
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Jorge Davila
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xvii, 111 p. : ill.
Abstract
(type = abstract)
MicroRNAs are key regulators of biological processes. In this thesis we identify mir-9 as a critical regulator during NSC proliferation and neuronal differentiation. Interestingly the role of mir-9 in NSCs differs depending on our experimental model. In a rat multipotential NSC, exogenous expression of mir-9 alone can enhance their neurogenic capacity. Meanwhile, in human NSCs mir-9 plays a role in NSC maintenance. These results either suggest that the role of mir-9 is not conserved across species or most likely that mir-9 can exert different cellular functions depending on the cellular context. In H1 NSCs subpopulations of cells expressing different combinations of proliferation and/or differentiation markers in cultures of neuronal differentiating cells were identified. The combined expression of differentiating and proliferating markers is a clear indication that differentiation is a “fluid” process that requires multiple overlapping steps to reach a specific phenotype. In addition, we identified OC2 as an anti-neuronal gene. Its mRNA is associated with the RISC complex during NSC neuronal differentiation where mir-9 serves to reduce OC2 protein output. These data together show that mir-9 serves to regulate NSCs in a context dependent manner and that it can canalyze neuronal differentiation by inhibiting genes that would prevent or retard neuronal differentiation.
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061184
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T39C6WS1
Genre
(authority = ExL-Esploro)
ETD doctoral
Back to the top
Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Davila
GivenName
Jorge
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2011-04-15 15:00:07
AssociatedEntity
Name
Jorge Davila
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2011-05-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2011-11-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after November 30th, 2011.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
Back to the top
Technical
FileSize
(UNIT = bytes)
3240960
OperatingSystem
(VERSION = 5.1)
windows xp
ContentModel
ETD
MimeType
(TYPE = file)
application/pdf
MimeType
(TYPE = container)
application/x-tar
FileSize
(UNIT = bytes)
3246080
Checksum
(METHOD = SHA1)
0a835cfe1913b71281f87974530520faef469e95
Back to the top
Statistical Profile