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Impact of wastewater effluent on the structure and antibiotic resistance of native microbial communities
Descriptive
TitleInfo
Title
Impact of wastewater effluent on the structure
and antibiotic resistance of native microbial communities
and antibiotic resistance of native microbial communities
Name
(type = personal)
NamePart
(type = family)
Honarbakhsh
NamePart
(type = given)
Maryam
NamePart
(type = date)
1980-
DisplayForm
MARYAM HONARBAKHSH
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Bini
NamePart
(type = given)
Elisabetta
DisplayForm
Elisabetta Bini
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Vetriani
NamePart
(type = given)
Costantino
DisplayForm
Costantino Vetriani
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Kerkhof
NamePart
(type = given)
Lee
DisplayForm
Lee Kerkhof
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2011
DateOther
(qualifier = exact);
(type = degree)
2011-10
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
In this work, we investigated the impact of antibiotic resistant bacteria from final effluents of a sewage discharge on the selection of antibiotic resistance in natural bacterial communities in sites downstream the treatment plant. Samples were collected from the final effluents of the wastewater treatment plant and from two sites along the receiving stream. A fourth site, upstream of the discharge, was used as control. Phylogenetic analysis of the 16S rRNA gene sequences was performed to derive the composition and structure of the four microbial communities. Clone library data and TRFLP profiles showed that the four communities were all dominated by Betaproteobacteria, which constituted approximately two thirds of the final effluent community. The diversity and abundance of other bacterial phylotypes varied across sites. Bacterial diversity included 9 phylotypes in the final effluent, 15 and 13 phylotypes respectively in the sites downstream the discharge. Furthermore, bacteria were isolated from the same samples using culture-dependent techniques, which allowed to link antibiotic resistance to particular bacterial species. While clone libraries were dominated by Betaproteobacteria, culturable isolates mainly belonged to the class of Gammaproteobacteria. Specifically, the analysis of the isolates from the final effluent showed the presence of Bacillus, Enterobacter, Acinetobacter, and Staphylococcus strains, while samples collected downstream from the plant were characterized by species belonging to the genera Brevibacterium, Chryseobacterium, Aeromonas and Delftia. All the isolates were resistant to amoxicillin, and most displayed resistance to multiple antibiotics. The distribution of β-lactamase genes across the four sites was also assessed. Phylogenetic analysis revealed that the distribution of the bla TEM4 gene is divided to two main clusters. The first cluster contains sequences that are exclusively detected at the plant and receiving water, but not at the upstream site, while sequences of the second cluster were present at all of the four sites.
Subject
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_3643
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
vii, 58 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Maryam Honarbakhsh
Subject
(authority = ETD-LCSH)
Topic
Drug resistance in microorganisms
Subject
(authority = ETD-LCSH)
Topic
Sewage disposal plants
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063455
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3F18XTF
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
HONARBAKHSH
GivenName
MARYAM
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2011-10-01 12:46:53
AssociatedEntity
Name
MARYAM HONARBAKHSH
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2011-10-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2013-10-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2013.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
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2372096
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