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Fabrication and evaluation of a tyrosine-derived polycarbonate conduit to enhance functional recovery of a 5 mm peripheral nerve gap in a mouse femoral nerve model
Descriptive
TitleInfo
Title
Fabrication and evaluation of a tyrosine-derived polycarbonate conduit to enhance functional recovery of a 5 mm peripheral nerve gap in a mouse femoral nerve model
Name
(type = personal)
NamePart
(type = family)
Ezra
NamePart
(type = given)
Mindy Iris
NamePart
(type = date)
1983-
DisplayForm
Mindy Ezra
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Kohn
NamePart
(type = given)
Joachim
DisplayForm
Joachim Kohn
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Shreiber
NamePart
(type = given)
David
DisplayForm
David Shreiber
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Cai
NamePart
(type = given)
Li
DisplayForm
Li Cai
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Schachner
NamePart
(type = given)
Melitta
DisplayForm
Melitta Schachner
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2012
DateOther
(qualifier = exact);
(type = degree)
2012-01
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Each year, over 200,000 people in the United States are treated for peripheral nerve injuries requiring surgery. Several nerve guidance conduits (NGCs) have been approved by the FDA, however, when used to repair critical size defects, regeneration results in limited functional recovery and poor quality. Therefore, a conduit fabricated from a material encouraging regeneration, specifically enhancing neurite outgrowth and functional recovery is required. Tyrosine-derived polycarbonates (TyrPCs) are biodegradable and biocompatible polymers offering a unique chemistry that allows for the optimization of their chemical, mechanical, and cellular properties for a specific application. These materials have been used in several medical devices and are effective at supporting neurite outgrowth in vitro. Additionally, peptide mimics of HNK-1 elucidated by the Schachner laboratory show significant promise when used in soluble form within conduits used to treat short defects. For critical size defects, soluble HNK-1 may not suffice; the mimic may diffuse away from the injury site. Thus, the goal of this research was twofold: 1) to develop a TyrPC NGC to treat critical size nerve defects and 2) to establish alternative methods of HNK-1 delivery. Three methods were explored: a collagen hydrogel filler grafted with HNK-1 (developed and provided by the Shreiber laboratory), the secretion of HNK-1 from genetically engineered stem cells, and slow release of HNK-1 from the NGC outer walls. TyrPC was compared to commercially available polyethylene in vitro and conduits fabricated from both materials were evaluated in the mouse femoral nerve model. In vitro results indicated greater protein adsorption and neurite outgrowth on TyrPC as compared to polyethylene. In vivo results showed improved functional recovery and quality of nerve regeneration in animals treated with TyrPC and suggested greater Schwann cell presence and fibrin matrix formation. Furthermore, in vitro results confirmed usefulness of 2 new methods for HNK-1 delivery, release from stem cells and the NGC itself. In vivo studies demonstrated that the influence of a collagen hydrogel with and without HNK-1 depends upon the TyrPC nerve conduit structure: whether or not the conduit was porous. In conclusion, conduits fabricated from TyrPC offer the potential for treatment of critical size nerve gaps.
Subject
(authority = RUETD)
Topic
Biomedical Engineering
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_3682
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xxi, 163 p. : ill.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Mindy Iris Ezra
Subject
(authority = ETD-LCSH)
Topic
Nerves, Peripheral--Regeneration
Subject
(authority = ETD-LCSH)
Topic
Polycarbonates--Therapeutic use
Subject
(authority = ETD-LCSH)
Topic
Polymers in medicine
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000064082
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3VQ31RH
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Ezra
GivenName
Mindy
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2011-10-05 10:18:21
AssociatedEntity
Name
Mindy Ezra
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-01-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2013-01-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after January 30th, 2013.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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5887488
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