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The relationship of meiotic checkpoint regulation, synapsis, and crossing over in Drosophila melanogaster
Descriptive
TitleInfo
Title
The relationship of meiotic checkpoint regulation, synapsis, and crossing over in Drosophila melanogaster
Name
(type = personal)
NamePart
(type = family)
White-Brown
NamePart
(type = given)
Sanese Kania
NamePart
(type = date)
1985-
DisplayForm
Sanese White-Brown
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Mckim
NamePart
(type = given)
Kim
DisplayForm
Kim Mckim
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Steward
NamePart
(type = given)
Ruth
DisplayForm
Ruth Steward
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Barr
NamePart
(type = given)
Maureen
DisplayForm
Maureen Barr
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2012
DateOther
(qualifier = exact);
(type = degree)
2012-05
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Proper chromosome segregation is achieved through three important meiotic events. The first is synapsis, where a proteinaceous structure, the synaptonemal complex (SC), forms between homologous chromosomes and juxtaposes them together. Then, recombination, which is initiated by programmed double strand breaks (DSBs) introduced into DNA that leads to a crossover event. Chiasmata, which are physical markers of where an exact exchange of genetic material occurred between homologous chromosomes during crossing over, are important to maintain the genetic integrity and variability of offspring. Zip3, a conserved meiotic protein found in budding yeast to humans, has been found to be required for crossing over. Furthermore, previous studies in budding yeast (Zip3) and C. elegans (ZHP-3) have shown the homologs to be crossover markers. However, the exact mechanism as to how Zip3 combines synapsis and recombination to promote crossing over is unknown. In Drosophila melanogaster, there are two homologs of Zip3, Zip3 related protein on the third and X chromosome (Z3rp3 and Z3rpX). Because Drosophila currently does not have a way to visualize crossovers, this study involves determining the function of Z3rp3 and Z3rpX during meiotic recombination and if they are crossover markers. HA-tagged transgenes were constructed to express the protein of each homolog. Z3rp3 HA and Z3rpX HA transgenes revealed an abnormal localization pattern, resulting in them not being good crossover markers. However, the transgenes displayed dominant negative effects on meiosis, indicating they had another important function on the process. Loss of function mutants created in z3rp3 and z3rpX showed that they not only had separate functions on the events of meiosis leading to proper chromosome segregation, but also that they seemed to be redundant, since the z3rpX z3rp3 double mutant exhibited the most severe phenotypes on synapsis, recombination, and crossing over. Overall, z3rp may be playing similar roles in the communication between SC formation and recombination events as in budding yeast and C. elegans, but in different contexts. Thus, I have results that provide new insights into the functional features of z3rp in Drosophila that confirms its role in crossing over.
Subject
(authority = RUETD)
Topic
Cell and Developmental Biology
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_3887
PhysicalDescription
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electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xii, 66 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Sanese Kania White-Brown
Subject
(authority = ETD-LCSH)
Topic
Drosophila melanogaster
Subject
(authority = ETD-LCSH)
Topic
Chromosomes--Analysis
Subject
(authority = ETD-LCSH)
Topic
Cell metabolism--Regulation
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065292
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3W094VF
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
White-Brown
GivenName
Sanese
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-04-09 17:51:09
AssociatedEntity
Name
Sanese White-Brown
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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