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The roles of cypin in brain-derived neurotrophic factor (BDNF)-promoted neuronal dendrite branching and in the regulation of postsynaptic density-95 (PSD-95) protein levels
Descriptive
TitleInfo
Title
The roles of cypin in brain-derived neurotrophic factor (BDNF)-promoted neuronal dendrite branching and in the regulation of postsynaptic density-95 (PSD-95) protein levels
Name
(type = personal)
NamePart
(type = family)
Kwon
NamePart
(type = given)
Munjin
NamePart
(type = date)
1981-
DisplayForm
Munjin Kwon
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Firestein
NamePart
(type = given)
Bonnie L
DisplayForm
Bonnie L Firestein
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
D'Arcangelo
NamePart
(type = given)
Gabriella
DisplayForm
Gabriella D'Arcangelo
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Millonig
NamePart
(type = given)
James H
DisplayForm
James H Millonig
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Dreyfus
NamePart
(type = given)
Cheryl F
DisplayForm
Cheryl F Dreyfus
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2012
DateOther
(qualifier = exact);
(type = degree)
2012-05
CopyrightDate
(qualifier = exact)
2012
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Alterations in dendrite branching and morphology are present in many neurodegenerative diseases. These variations disrupt postsynaptic transmission and affect neuronal communication. Thus, it is important to understand the molecular mechanisms that regulate dendritogenesis and how they go awry during disease states. Previously, our laboratory showed that cypin, a guanine deaminase, increases dendrite number by regulating microtubule assembly. Cypin interacts with postsynaptic density-95 (PSD-95) protein, and reduces its synaptic clustering in cultured neurons when overexpressed. Brain-derived neurotrophic factor (BDNF) is one of most studied neurotrophins, which play a role in regulating neurite growth and branching in neurons. Here we examined the complex actions of cypin in BDNF-promoted dendrite branching and in regulating PSD-95 protein levels. First, we show the expression patterns of cypin and its new isoform in the developing rat brain. Second, we report that treatment of neurons with BDNF results in different effects, depending on how it is administered. Global administration of BDNF increases cypin mRNA and protein levels to regulate dendrite number only in the region proximal to the cell body. BDNF increases cypin levels via cyclic adenosine 3’5’-monophosphate (cAMP) response element-binding protein (CREB)-dependent transcriptional regulation by mitogen-activated protein kinase (MAPK) signaling pathways. Furthermore, localized BDNF application to the dendritic arbor also increases dendrite branching at sites of stimulation. Interestingly, local administration of BDNF increases dendrite branching not only proximal to the soma but also at distal regions in the arbor. Taken together, these studies suggest that distinct sources of BDNF regulate dendritic arborization via different mechanisms. Finally, we investigate the role of cypin on the expression of its binding partner, PSD-95. Overexpression of cypin increases total cellular PSD-95 levels, and this effect is occluded by treatment with a proteasome inhibitor. Since PSD-95 is important for assembling signaling complexes and mediating synaptic activity, a role for cypin at synaptic sites in proteasome-dependent regulation of PSD-95 is proposed. Therefore, we suggest new roles of cypin in BDNF-promoted dendrite branching and in the alteration of PSD-95 protein levels at synaptic sites.
Subject
(authority = RUETD)
Topic
Cell and Developmental Biology
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_3897
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xi, 148 p. : ill.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Munjin Kwon
Subject
(authority = ETD-LCSH)
Topic
Nervous system--Degeneration
Subject
(authority = ETD-LCSH)
Topic
Dendrites
Subject
(authority = ETD-LCSH)
Topic
Enzymes--Regulation
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065177
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3B85716
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Kwon
GivenName
Munjin
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-04-11 13:37:07
AssociatedEntity
Name
Munjin Kwon
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-05-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2014-05-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2014.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
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(UNIT = bytes)
4628480
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windows xp
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ETD
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application/pdf
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application/x-tar
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4628480
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