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Characterization of two Physcomitrella patens Caffeoyl coenzyme A O-methyltransferase-like proteins
Descriptive
TitleInfo
Title
Characterization of two Physcomitrella patens Caffeoyl coenzyme A O-methyltransferase-like proteins
Name
(type = personal)
NamePart
(type = family)
Pilipetskii
NamePart
(type = given)
Sergei
NamePart
(type = date)
1983-
DisplayForm
Sergei Pilipetskii
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Belanger
NamePart
(type = given)
Faith
DisplayForm
Faith Belanger
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Lawton
NamePart
(type = given)
Michael
DisplayForm
Michael Lawton
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Zilinskas
NamePart
(type = given)
Barbara
DisplayForm
Barbara Zilinskas
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2013
DateOther
(qualifier = exact);
(type = degree)
2013-01
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Lignin is one of the most abundant biopolymers. It accounts for up to 25% of plant biomass in the biosphere. Lignified cell walls represent an important evolutionary step in plant adaptation to land life. This adaptation provided structural support, nutrient transport, protection from UV radiation, desiccation stress and protection from herbivores and pathogens. Due to lignin’s long half-life, it has become a major carbon sink, causing depletion of the CO2 levels and an increase in O2 levels. Currently lignin research is important for the forage and biofuel industries. It has been seen that a reduction in lignin content leads to higher digestibility and higher release of cellulose from plants. Caffeoyl coenzyme A O-methyltransferases (CCoAOMTS) are a class of enzymes that transfer a methyl group from S-adenosylmethionine to an alcohol group. These enzymes are necessary for lignin production in plants. Previous work has shown that knocking down the function of this enzyme in higher plants caused a decrease in lignin content. Physcomitrella patens is a moss, which is considered not to synthesize lignin but it contains genes for the full lignin biosynthesis pathway. Currently there is some evidence of lignin-like compounds occurring in moss, but evidence of a truly lignified cell wall does not exist. This study looks at two specific enzymes from Physcomitrella patens, Pp-OMT1 and Pp-OMT2. These enzymes resemble CCoAOMTs. Both enzymes were tested for substrate specificity and compared to a true caffeoyl coenzyme A O-methyltransferase from Arabidopsis thalina, AtCCoAOMT1. Furthermore kinetics of Pp-OMT2 and AtCCoAOMT1 were compared. Our results showed that Pp-OMT2 is likely to be a true CCoAOMT while Pp-OMT1 is not.
Subject
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Identifier
ETD_4499
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000067822
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
vii, 33 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Sergei Pilipetskii
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Subject
(authority = ETD-LCSH)
Topic
Lignin--Research
Subject
(authority = ETD-LCSH)
Topic
Biopolymers
Identifier
(type = doi)
doi:10.7282/T38C9TZ8
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Pilipetskii
GivenName
Sergei
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2013-01-08 09:28:22
AssociatedEntity
Name
Sergei Pilipetskii
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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