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Genetic studies for selenate and tellurate reduction processes in facultative bacteria

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TitleInfo
Title
Genetic studies for selenate and tellurate reduction processes in facultative bacteria
Name (type = personal)
NamePart (type = family)
Theisen
NamePart (type = given)
Joanne
NamePart (type = date)
1989-
DisplayForm
Joanne Theisen
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Yee
NamePart (type = given)
Nathan
DisplayForm
Nathan Yee
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Reinfelder
NamePart (type = given)
John
DisplayForm
John Reinfelder
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Strom
NamePart (type = given)
Peter
DisplayForm
Peter Strom
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2013
DateOther (qualifier = exact); (type = degree)
2013-01
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
The bioavailability and toxicity of selenium in the environment is strongly affected by redox transformations. In this study, we conducted spectroscopic, genetic, and macroscopic chemical measurements to investigate the ability of Citrobacter freundii to catalyze the reduction of soluble selenate oxyanions [Se(VI)] to poorly soluble elemental selenium [Se(0)]. The results indicate that C. freundii forms a red precipitate on selenate containing agar after 48 hours of incubation. In liquid culture, bulk chemical measurements show the removal of selenate oxyanions from solution only after oxygen is completely removed from the bacterial media. Selenate reduction by C. freundii is a substrate specific process and does not simply bind or absorb to cells, as it does not reduce arsenic under the same conditions. X-ray absorption near edge spectra analysis of cell pellets collected from selenate incubations after 7 days show the formation of solid-phase elemental selenium. PCR amplification and DNA sequencing revealed that C. freundii carries the ynfEGH operon, a gene cluster in the DMSO reductase family previously shown to be responsible for selenate reduction in other gamma proteobacteria. Sequence analysis of ynfEGH operon possess a FNR binding site (Fumarate nitrate reduction regulator), and twin-arginine translocation (Tat) signal sequence, The environmental implications of selenate reduction by C. freundii for bioremediation purposes are discussed. The genetic identity and co-factor composition of the bacterial tellurate reductase are currently unknown. In this study, we examined the requirement of molybdopterin biosynthesis and molybdate transporter genes for tellurate reduction by Escherichia coli. The results demonstrate that mutants carrying deletions of the moaA, moaB, moaE, or mog gene in molybdopterin biosynthesis pathway lost the ability to reduce tellurate. Deletion of the modB or modC genes in molybdate transport pathway also resulted in complete loss of tellurate reduction activity. Genetic complementation by the wild-type sequences restored tellurate reduction activity in the mutant strains. These findings provide genetic evidence that the tellurate reductase in E. coli is a molybdopterin-containing enzyme.
Subject (authority = RUETD)
Topic
Environmental Sciences
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_4414
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
viii, 46 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Joanne Theisen
Subject (authority = ETD-LCSH)
Topic
Selenosis
Subject (authority = ETD-LCSH)
Topic
Aerobic bacteria
Subject (authority = ETD-LCSH)
Topic
Selenium
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000067849
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3Z31XC9
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Theisen
GivenName
Joanne
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2012-12-11 12:30:22
AssociatedEntity
Name
Joanne Theisen
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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