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Iron oxide reduction by a clostridial consortium

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TitleInfo
Title
Iron oxide reduction by a clostridial consortium
SubTitle
insights from physiological and genome analyses
Name (type = personal)
NamePart (type = family)
Shah
NamePart (type = given)
Madhavi
NamePart (type = date)
1986-
DisplayForm
MADHAVI SHAH
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Yee
NamePart (type = given)
Nathan
DisplayForm
Nathan Yee
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Barkay
NamePart (type = given)
Tamar
DisplayForm
Tamar Barkay
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
co-chair
Name (type = personal)
NamePart (type = family)
Reinfelder
NamePart (type = given)
John
DisplayForm
John Reinfelder
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Strom
NamePart (type = given)
Peter
DisplayForm
Peter Strom
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2013
DateOther (qualifier = exact); (type = degree)
2013-05
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Iron reducing organisms are ubiquitous and phylogenetically diverse. Their acitivity in the environment not only affects the speciation of iron in aquatic systems or sediments, but it plays a major role in iron mineral formation, sediment diagenesis, carbon cycling and the fate and transport of contaminants in the subsurface environment. Iron oxide reduction has been extensively studied with pure cultures of dissimilatory iron reducers such as Geobacter and Shewanella. However, the effects of syntrophy on iron oxide reduction and secondary mineralization by microbial consortia are poorly understood. The research presented in this dissertation describes enrichment of an iron reducing anaerobic microbial consortium from subsurface sediments. The consortium was composed of fermentative Clostridium sp. strain FGH and a novel Veillonellaceae, strain RU4. The experimental results indicate the role of hydrogen, sulfate and growth medium in rapid reductive dissolution of iron oxides and subsequent secondary mineralization by the clostridial consortium. The data demostrated that iron oxide reduction by the consortium was catalyzed by both biotic reduction by strain FGH and syntrophy driven biotic/abiotic reduction by strain RU4. Reductive dissolution of iron oxides by the consortium resulted in formation of solid-phase Fe(II) and poorly crystalline ferrous bearing minerals such as nanoparticulate magnetite and iron sulfides. The results of this work provide new insights in the ecological role of Clostridia in subsurface Fe(II) mineral formation processes. Unlike iron respiring Geobacter and Shewanella, the mechanism of iron oxide reduction is poorly understood in iron reducing fermentative bacteria. In this study we conducted experiments with fermentative Clostridium sp. strain FGH to elucidate its mode of iron reduction. Experiments and genome analysis suggest an indirect, cytochrome c independent mechanism of iron reduction by strain FGH. Veillonellaceae are recently found to be active during bioremediation studies at contaminanted sites. Genomic characterization of the novel Veillonellaceae, strain RU4, that could not be isolated in pure culture revealed its potential metabolic capabilities. The strain RU4 draft genome consists of fatty acid metabolism genes and pathways for sulfate, sulfite and polysulfide reduction. These results may assist in better understanding of the biogeochemical and ecological role of this novel subsurface bacterium.
Subject (authority = RUETD)
Topic
Microbial Biology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_4662
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xii, 114 p. : ill.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Madhavi Shah
Subject (authority = ETD-LCSH)
Topic
Iron oxides
Subject (authority = ETD-LCSH)
Topic
Iron--Analysis
Subject (authority = ETD-LCSH)
Topic
Reduction (Chemistry)
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000068966
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T33R0RFJ
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
SHAH
GivenName
MADHAVI
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2013-04-12 16:33:45
AssociatedEntity
Name
MADHAVI SHAH
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2013-05-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2015-05-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2015.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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