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The application of a passive dosing system for determining zebrafish early life stage toxicity of hydrocarbons for use in calibrating a predictive model to acute and chronic endpoints
Descriptive
TitleInfo
Title
The application of a passive dosing system for determining zebrafish early life stage toxicity of hydrocarbons for use in calibrating a predictive model to acute and chronic endpoints
Name
(type = personal)
NamePart
(type = family)
Butler
NamePart
(type = given)
Joshua D.
NamePart
(type = date)
1984-
DisplayForm
Joshua Butler
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Cooper
NamePart
(type = given)
Keith R
DisplayForm
Keith R Cooper
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Reinfelder
NamePart
(type = given)
John R
DisplayForm
John R Reinfelder
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Rodenburg
NamePart
(type = given)
Lisa A
DisplayForm
Lisa A Rodenburg
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Parkerton
NamePart
(type = given)
Thomas F
DisplayForm
Thomas F Parkerton
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2013
DateOther
(qualifier = exact);
(type = degree)
2013-10
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
The target lipid model has been used to derive water quality objectives that are intended to be protective of chronic effects posed by hydrocarbons. However, experimental Early Life Stage toxicity data for fish are limited and further data are needed to confirm model predictions. Efforts are underway to develop a zebrafish embryo toxicity test guideline to reduce, refine and replace the use of vertebrates in animal testing. An adaptation of this method which includes embryo lethal and sub-lethal developmental endpoints after a 5 day exposure as well as larval survival and growth endpoints during a subsequent 25 day test period is described. To deliver well controlled exposure concentrations, a passive dosing system consisting of silicone coated vials and silicone O-rings was employed. This research consisted of three phases. During the first phase, phenanthrene was used as a reference substance to test efficacy of a passive dosing design as well as compare observed effect data with published effect data. Concentrations shown to cause adverse effects in this phase of the research were in the range of previous studies that have investigated the chronic effects of phenanthrene on fish. In the second phase, separate exposures to five aromatic hydrocarbons (AHs) including 1-methylnaphthalene, phenanthrene, 1, 2, 3, 4, 5, 6, 7, 8, octahydrophenanthrene, benzo(a)pyrene and chrysene utilizing the passive dosing system and ELS design were investigated. Subsequently, during phase two, six short-term (120 hour) exposures for naphthalene, 1-methylnaphthalene, biphenyl, phenanthrene, pyrene and 1, 2, 3, 4, 5, 6, 7, 8, - octahydrophenanthrene were also employed. Data collected from acute and chronic exposures were used to estimate the acute and chronic critical target lipid body burdens for zebrafish using the TLM framework. During the third phase a simple mixture of ten saturated, unsaturated, and aromatic hydrocarbons with a KOW range of ca. 4 - 7.5 was tested during a 30 day ELS test. Three treatment levels were selected to provide concentrations that corresponded to a toxic unit range above and below the predicted effect concentration assuming concentration addition. Observed acute and chronic effects with the mixture were consistent with the assumption of additive toxicity.
Subject
(authority = RUETD)
Topic
Environmental Sciences
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_4992
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xv, 139 p. : ill.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Joshua D. Butler
Subject
(authority = ETD-LCSH)
Topic
Hydrocarbons--Toxicity testing
Subject
(authority = ETD-LCSH)
Topic
Zebra danio--Toxicology
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T38W3BB9
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Butler
GivenName
Joshua
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2013-09-11 14:05:47
AssociatedEntity
Name
Joshua Butler
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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