Wang, Junjie. Interaction of the components in human and escherichia coli pyruvate dehydrogenase multienzyme complexes. Retrieved from https://doi.org/doi:10.7282/T3FX77J5
DescriptionThe pyruvate dehydrogenase multienzyme complex (PDHc) plays crucial physiological roles in human. Structural study of this complex with traditional methods is highly challenging due to its large size (> 10 M Da). In this thesis, the Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS) method was employed to study the protein-protein interactions of the components in both E. coli and human PDHc, which successfully revealed their binding loci, as well as the active center coupling, the substrate coupling, the allosteric regulation, and the catalytic mechanisms. The findings greatly improved the current understanding of the PDHc model, creating opportunities for designing new drugs targeting protein-protein interactions.