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Changes in BMP and EGFR signaling components underlie the evolution of Drosophila eggshell morphologies

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TitleInfo
Title
Changes in BMP and EGFR signaling components underlie the evolution of Drosophila eggshell morphologies
Name (type = personal)
NamePart (type = family)
Niepielko
NamePart (type = given)
Matthew Gene
NamePart (type = date)
1985-
DisplayForm
Matthew Niepielko
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Yakoby
NamePart (type = given)
Nir
DisplayForm
Nir Yakoby
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Piccoli
NamePart (type = given)
Benedetto
DisplayForm
Benedetto Piccoli
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Shvartsman
NamePart (type = given)
Stanislav Y
DisplayForm
Stanislav Y Shvartsman
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Camden Graduate School
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2014
DateOther (qualifier = exact); (type = degree)
2014-05
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
A fundamental requirement for understanding the evolution of tissue morphogenesis involves exploring underlying changes in cell signaling amongst species. We used the magnificent diversity of Drosophila eggshell morphologies to investigate mechanisms guiding morphological variation. Drosophila eggshell structures, including the dorsal appendages (DAs) and the dorsal ridge (DR), are formed during oogenesis when a 2D monolayer of follicle cells (FCs) overlying the oocyte is instructed by numerous cell signaling pathways to form the eggshell. Here, we focus on the bone morphogenetic protein (BMP) signaling in reference to DAs formation, and the epidermal growth factor receptor (EGFR) in reference to DR formation. BMP signaling in the FCs is initiated when the anteriorly secreted transforming growth factor-β-like Decepentaplegic (DPP) activates a uniform and then patterned type I BMP receptor Thickveins (TKV). We found that the pattern of BMP signaling is dynamic and spatially diverse in species with different DAs morphologies. Using computational modeling and experimental validation, we found that qualitative and quantitative changes in TKV can account for different BMP signaling outputs across species. The DR is a lumen-like structure along the dorsal-most side on eggshells of numerous species and absent from D. melanogaster eggshells. We developed a binary matrix to analyze 180 2D expression patterns of a family of structural proteins, the Chorion proteins, in species with and without a DR and associate DR patterns to domains of known regulation. The DR domain clusters with EGFR regulated domains and thus, we focused on EGFR and DR formation. EGFR activation begins when the transforming growth factor-α-like ligand, Gurken (GRK), is secreted from the oocyte and creates a dorsal-ventral activation gradient. We found that DR morphologies correlate with EGFR signaling and GRK patterns in the DR domain. In the DR species D. willistoni, we successfully perturbed EGFR signaling and DR formation by genetically perturbing GRK levels. Expression of D. willistoni GRK (wGRK) in D. melanogaster rescues the GRK null phenotype and in some cases, wGRK is sufficient to produce a ridge-like structure on D. melanogaster eggshells. Our results support the idea that changes in major components of developmental pathways underlie the evolution of morphologies.
Subject (authority = RUETD)
Topic
Computational and Integrative Biology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5651
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xiii, 78 p. : ill.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Matthew Gene Niepielko
Subject (authority = ETD-LCSH)
Topic
Drosophila--Morphogenesis
Subject (authority = ETD-LCSH)
Topic
Bone morphogenetic proteins
RelatedItem (type = host)
TitleInfo
Title
Camden Graduate School Electronic Theses and Dissertations
Identifier (type = local)
rucore10005600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T37S7M09
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Niepielko
GivenName
Matthew
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-05-01 17:51:56
AssociatedEntity
Name
Matthew Niepielko
Role
Copyright holder
Affiliation
Rutgers University. Camden Graduate School
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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