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Enteroid culture and its impact on stem cell and cancer research
Descriptive
TitleInfo
Title
Enteroid culture and its impact on stem cell and cancer research
Name
(type = personal)
NamePart
(type = family)
Hoffman
NamePart
(type = given)
Andrew
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Andrew Hoffman
Role
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author
Name
(type = personal)
NamePart
(type = family)
Millonig
NamePart
(type = given)
James
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James Millonig
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Verzi
NamePart
(type = given)
Michael
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Michael Verzi
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Steward
NamePart
(type = given)
Ruth
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Ruth Steward
Affiliation
Advisory Committee
Role
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internal member
Name
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Gu
NamePart
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Sam
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Sam Gu
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
(qualifier = exact)
2014
DateOther
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(type = degree)
2014-05
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
The technique of enteroid cell culture has had an enormous effect on the study of intestinal stem cells as well as colorectal cancer, and will continue to aid scientists in making advances in these fields in the future. In this thesis, the intestinal epithelium is introduced and the cell types within it are explained, stating why it is so crucial to understand its properties. The brief history of enteroid culture is gone over, beginning with its inception in 2009 and the expansion and initial discoveries made with the technique up until and including 2012. Next, the main section of the thesis includes detailed protocols on how to extract intestinal crypts and grow enteroid cultures, as well as perform some modifying techniques such as lentiviral infection and the creation of enterospheres. My research and contribution to Dr. Verzi’s lab was in the perfecting of these protocols. Finally, the thesis concludes with the most recent discoveries and advances made using the enteroid culture technique, and its potential for future innovation and discovery.
Subject
(authority = RUETD)
Topic
Cell and Developmental Biology
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_5450
PhysicalDescription
Form
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electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
iv, 48 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Andrew Hoffman
Subject
(authority = ETD-LCSH)
Topic
Cell culture
Subject
(authority = ETD-LCSH)
Topic
Epithelium
Subject
(authority = ETD-LCSH)
Topic
Colon (Anatomy)--Cancer--Research
Subject
(authority = ETD-LCSH)
Topic
Stem cells--Research
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3ZC8158
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Hoffman
GivenName
Andrew
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2014-04-11 11:50:27
AssociatedEntity
Name
Andrew Hoffman
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
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windows xp
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