Changes to the gut microflora resulting from a number of environmental influences are well documented. (123) Enteric pathogens have been shown to promote a shift away from the predominance of Gram negative toward Gram-positive species however, evidence of Noroviruses initiating such changes has only recently been documented. (90, 135) Noroviruses (NoV) are the most common cause of gastroenteritis in the world accounting for about 50% of all outbreaks of foodborne illness and more than 90% of outbreaks with a non-bacterial origin worldwide (97, 108). Generally self-limiting, nausea, vomiting, and diarrhea are common symptoms of the illness (80). Nearby surfaces may become contaminated with aerosolized fecal matter or vomitus from being ill. These contaminated surfaces may be involved in the propagation of an outbreak as well as provide a surface that investigators may swab to obtain a sample for PCR amplification, which is the preferred method of NoV detection by the CDC (15). However, concerns about false outcomes resulting from contamination or amplification of inactivated genomic material leave room for supporting diagnostic methods to be investigated. Bacterial indicators have been used in the past to indicate the presence of fecal contamination and various indicators have been investigated to predict the presence of specific pathogens. Utilizing surface contamination and the change to the gut microbiome upon NoV infection could provide a new diagnostic technique for NoV diagnosis. The first step to creating this new diagnostic would be investigating the survival of the relevant bacterial species on surfaces of interest. It was the purpose of this thesis to study the survival of E. faecalis and B. fragilis on stainless steel, ceramic, glass, and polystyrene. E. faecalis survived above the detection limit for 16 weeks on all surfaces except ceramic on which, it was able to survive only 8 weeks above the detection limit. B. fragilis was able to survive 48 hours on all surfaces tested.
Subject (authority = RUETD)
Topic
Nutritional Sciences
Subject (authority = ETD-LCSH)
Topic
Intestines--Microbiology
Subject (authority = ETD-LCSH)
Topic
Enterococcus faecalis
Subject (authority = ETD-LCSH)
Topic
Bacteroides
Subject (authority = ETD-LCSH)
Topic
Gastroenteritis
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5767
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (viii, 53 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Hannah Kathleen Bolinger
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.