Homologous recombination sites during drosophila female meiosis and the role of the drosophila ino80 complex in meiotic recombination
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Cardona, Cristina Ochoa.
Homologous recombination sites during drosophila female meiosis and the role of the drosophila ino80 complex in meiotic recombination. Retrieved from
https://doi.org/doi:10.7282/T35X27CJ
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TitleHomologous recombination sites during drosophila female meiosis and the role of the drosophila ino80 complex in meiotic recombination
Date Created2014
Other Date2014-10 (degree)
Extent1 online resource (ix, 248 p. : ill.)
DescriptionDNA Double Strand breaks (DSBs) are one of the most lethal types of genomic damage. Irregularities in the repairs of DSBs can lead to chromosomal aberrations, such as deletions, translocations, and inversions that ultimately are responsible for cancer, infertility, and birth defects. Cells have several conserved and controlled DNA repair pathways, in which a plethora of factors must be activated and recruited to the break. Moreover, eukaryotes must also locally and transiently modify chromatin to allow access of repair factors. Very little is known about chromatin remodeling during DSB processing in higher eukaryotes, partially due to the lack of suitable study models. Here I used Drosophila female meiosis as the model and show the development of a marker and fixation protocol that enables the labeling and following of DSB sites during recombinational repair. Moreover, I provide evidence that early and late repair sites correspond to non-crossovers (NCO) and crossovers (CO), respectively. In the next part of this study, I provide novel evidence that: 1) COs persist longer than NCOs and are mobilized to the nuclear periphery, 2) movement of COs to the periphery is developmentally controlled, depends on ATR, and occurs during mid-pachytene when NCOs are repaired, 3) any persisting DSB can form MRN foci that are mobilized to the periphery from mid-pachytene on. Furthermore, I focus on the role of dINO80, an ATP-dependent chromatin remodeler, during meiotic recombination. To date it has never been shown that INO80 has a role in meiosis or whether it functions in early recognition steps. Here I show that Ino80, the catalytic subunit of the INO80 complex, interacts in a damage dependent fashion with components of the MRN complex, and that Ino80 is required for the recognition of the majority of meiotic DSBs. Furthermore this study suggests that the ATP-dependent remodeling function of dIno80 is crucial for its role in recognition of meiotic DSBs. Drosophila female meiosis in conjunction with the developed techniques in this study provide a powerful system to study the dynamics of meiotic DSB sites and to spatially and temporally dissect the role of chromatin modifying complexes in DSB repair.
NotePh.D.
NoteIncludes bibliographical references
Noteby Cristina Ochoa Cardona
Genretheses, ETD doctoral
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.