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Optogenetic, voltammetric, and histochemical characterization of striatal tyrosine hydroxylase interneurons

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Title
Optogenetic, voltammetric, and histochemical characterization of striatal tyrosine hydroxylase interneurons
Name (type = personal)
NamePart (type = family)
Xenias
NamePart (type = given)
Harry S.
NamePart (type = date)
1970-
DisplayForm
Harry S. Xenias
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Abercrombie
NamePart (type = given)
Elizabeth D
DisplayForm
Elizabeth D Abercrombie
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Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Tepper
NamePart (type = given)
James M
DisplayForm
James M Tepper
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Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Pare
NamePart (type = given)
Denis
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Denis Pare
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Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Creese
NamePart (type = given)
Ian
DisplayForm
Ian Creese
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Koós
NamePart (type = given)
Tibor
DisplayForm
Tibor Koós
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Wightman
NamePart (type = given)
Robert M
DisplayForm
Robert M Wightman
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = personal)
NamePart (type = family)
Rice
NamePart (type = given)
Margaret E
DisplayForm
Margaret E Rice
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - Newark
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2014
DateOther (qualifier = exact); (type = degree)
2014-10
CopyrightDate (encoding = w3cdtf)
2014
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
The traditional view of striatal dopamine originating from the midbrain remained unchallenged until a publication by Dubach et al. (1987) showed that in primates there are striatal interneurons immunoreactive for tyrosine hydroxylase (TH), the rate-limiting enzyme for catecholamine synthesis. It has generally been assumed in subsequent publications that striatal TH interneurons (THINs) are an intrinsic striatal source of dopamine (DA). The possibility that THINs could be DAergic has deep implications for both normal and pathological states, such as Parkinson’s disease. However, despite nearly three decades since the first report of striatal TH+ neurons, no direct evidence that THINs are DAergic or can compensate for the loss of nigrostriatal DA has been reported. To examine if THINs contain and release DA, two bacterial artificial chromosome (BAC) transgenic mouse lines were used that express either the Cre recombinase enzyme or the enhanced green fluorescent protein (EGFP) reporter. Both transgenes are under the control of the regulatory elements of TH and each respective mouse line is referred to as TH-Cre or EGFP-TH. Immunofluorescent cytochemistry revealed no colocalization of EGFP+ striatal cells with DA in TH-EGFP mice. Neither was there any colocalization of EGFP+ striatal cells with aromatic L-amino acid decarboxylase (AADC), the vesicular monoamine transporter-2 (VMAT2), or the dopamine transporter (DAT). Additionally, TH-Cre mice that were unilaterally lesioned by 6-OHDA in the midbrain were also bilaterally injected into the striatum with Cre-dependent recombinant adeno-associated virus (AAV), encoding the opsin protein channelrhodopsin-2 (ChR2) fused to the enhanced yellow fluorescent protein (EYFP) to transduce THINs to express ChR2. THINs were then light-activated by 2-5 ms blue light pulses during simultaneous fast-scan cyclic voltammetry (FSCV) and found to not release any detectable amounts of DA, even in the presence of nomifensine, a potent DA reuptake inhibitor. Lastly, it was found that THINs are powerfully modulated by DA and provide a widespread and powerful inhibition on SPNs. THINs then, despite containing TH, are not DAergic but a novel class of GABAergic interneuron that regulates the timing of the output responses of SPNs and add to the complexity of the striatal microcircuitry.
Subject (authority = RUETD)
Topic
Behavioral and Neural Sciences
Subject (authority = ETD-LCSH)
Topic
Dopamine
Subject (authority = ETD-LCSH)
Topic
Interneurons
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5924
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xxii, 164 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = vita)
Includes vita
Note (type = statement of responsibility)
by Harry S. Xenias
RelatedItem (type = host)
TitleInfo
Title
Graduate School - Newark Electronic Theses and Dissertations
Identifier (type = local)
rucore10002600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3W66NDR
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Xenias
GivenName
Harry
MiddleName
S.
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-09-27 01:23:47
AssociatedEntity
Name
Harry Xenias
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - Newark
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License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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ETD
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windows xp
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