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Vibrational spectroscopy and microscopic imaging of skin
Descriptive
TitleInfo
Title
Vibrational spectroscopy and microscopic imaging of skin
SubTitle
I. collagen hydration in type i collagen and dermal tissue II. lateral diffusion of exogenous agents in human SC III. ceramide permeation into human skin
Name
(type = personal)
NamePart
(type = family)
Zhang
NamePart
(type = given)
Qihong
DisplayForm
Qihong Zhang
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Mendelsohn
NamePart
(type = given)
Richard
DisplayForm
Richard Mendelsohn
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Huskey
NamePart
(type = given)
Phillip
DisplayForm
Phillip Huskey
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Lalancette
NamePart
(type = given)
Roger
DisplayForm
Roger Lalancette
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Walters
NamePart
(type = given)
Russel
DisplayForm
Russel Walters
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - Newark
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2014
DateOther
(qualifier = exact);
(type = degree)
2014-10
CopyrightDate
(encoding = w3cdtf)
2014
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Confocal Raman spectroscopy and dynamic vapor sorption (DVS) measurements of human Type I collagen and pigskin dermis were performed to probe relative humidity (RH) dependent differences in the nature and level of collagen hydration. Raman spectra were also acquired as a function of time for both Type I collagen and pigskin dermis samples upon exchange of a 100% RH H2O to deuterium oxide (D2O) environment. Alterations in Amide I and III modes were consistent with anticipated changes in hydrogen bonding strength as RH increased and upon H → D exchange. Of note is the identification of a Raman spectral marker (band at 938 cm-1) which appears to be sensitive to alterations in collagen-bound water. Analysis of DVS isotherms provided a quantitative measure of adsorbed and absorbed water vapor consistent with the Raman results. Infrared (IR) spectroscopic imaging was utilized to evaluate lateral diffusion in stratum corneum (SC) and elucidate intermolecular interactions between exogenous agents and SC constituents. In separate experiments, acyl chain perdeuterated oleic acid (OA-d) and deuterated dimethyl sulfoxide (DMSO-d) were applied to the surface of isolated human SC. The lateral distribution of permeant concentrations was monitored using the time-dependence of IR images. Diffusion coefficients (D) were estimated from Fick’s second law. Networked glyphs served as the major pathway for lateral distribution of OA-d. In glyph-poor regions, D values 0.3-1 x 10-8 cm2/s bracketed the OA-d data and apparently decreased with time and diffusion of DMSO-d suggests D values of ~10-7 cm2/s. Interactions between the exogenous agents and the SC were tracked from changes in CD2 and Amide I stretching frequencies. OA-d spectral changes suggest penetration into the ordered lipids of the SC; DMSO-d penetration results in perturbation of SC keratin structure. IR imaging was also used to investigate permeation properties of two types of ceramides in human skin. IR images were acquired after incubating for 24 or 48 H with deuterated ceramide suspended in oleic acid. IR images of ceramide concentration were obtained after applying Beer’s Law. Analysis of IR spectra and concentration images suggest the detection limit of both ceramide around 3.5 mM, and most of the ceramide reside in concentrated localized pockets on skin surface, and in deep glyphs. Further analysis indicates the permeation of both ceramide into top layers of SC.
Subject
(authority = RUETD)
Topic
Chemistry
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_5796
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xvii, 111 p. : ill.)
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Subject
(authority = ETD-LCSH)
Topic
Fourier transform infrared spectroscopy
Subject
(authority = ETD-LCSH)
Topic
Skin--Physiology
Note
(type = statement of responsibility)
by Qihong Zhang
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - Newark Electronic Theses and Dissertations
Identifier
(type = local)
rucore10002600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T30003QR
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Zhang
GivenName
Qihong
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2014-08-26 11:42:01
AssociatedEntity
Name
Qihong Zhang
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - Newark
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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