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Analysis of localization and trafficking of TrkB after BDNF treatment in rat hippocampal neurons
Descriptive
TitleInfo
Title
Analysis of localization and trafficking of TrkB after BDNF treatment in rat hippocampal neurons
Name
(type = personal)
NamePart
(type = family)
Williams
NamePart
(type = given)
Danaé
DisplayForm
Danaé Williams
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Friedman
NamePart
(type = given)
Wilma
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Wilma Friedman
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Kim
NamePart
(type = given)
Haesan
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Haesan Kim
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Gao
NamePart
(type = given)
Nan
DisplayForm
Nan Gao
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - Newark
Role
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(authority = RULIB)
school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
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(qualifier = exact)
2014
DateOther
(qualifier = exact);
(type = degree)
2015-01
CopyrightDate
(encoding = w3cdtf);
(qualifier = exact)
2014
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Neurotrophins regulate neuronal cell survival and death in hippocampal neurons via two distinct receptors, TrkB, a member of the Trk family of receptor tyrosine kinases and the p75, neurotrophin receptor (p75NTR). When BDNF binds to TrkB, the association with p75NTR is enhanced. Yet, the purpose of the association of these two receptors is unknown. This thesis investigated whether the association with p75NTR directs the trafficking of TrkB to specific endosomal compartments. To determine whether the treatment with BDNF in the presence or absence of p75NTR alters the trafficking of TrkB though the endosomal pathway, we examined and analyzed the localization of TrkB and pAkt, a downstream effector of the PI3K pathway, in wild type and p75-/- rat hippocampal neurons using immunocytochemistry and confocal microscopy. Our data suggested that the addition of BDNF increased trafficking of pTrkB to the early and recycling endosomes. However, in the absence of p75NTR, more TrkB appeared to traffic to the lysosome, suggesting that p75NTR may participate in directing trafficking of TrkB to the endosomal pathway and preventing its degradation. Additionally, activation of pAkt localized in the late and recycling endosomes in the wild type neurons. These results suggested that p75NTR may direct the internalized TrkB through the endosomal pathway.
Subject
(authority = RUETD)
Topic
Biology
Subject
(authority = ETD-LCSH)
Topic
Neurotrophic functions
Subject
(authority = ETD-LCSH)
Topic
Neurons--Growth
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_6070
PhysicalDescription
Form
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electronic resource
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application/pdf
InternetMediaType
text/xml
Note
Supplementary File: Appendix of Analysis of localization and trafficking of TrkB after BDNF treatment in rat hippocampal neurons
Extent
1 online resource (vii, 22 p.)
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Danaé Williams
RelatedItem
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TitleInfo
Title
Graduate School - Newark Electronic Theses and Dissertations
Identifier
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rucore10002600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3RF5WQG
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Williams
GivenName
Danaé
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2014-12-15 21:47:15
AssociatedEntity
Name
Danae Williams
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - Newark
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
RULTechMD
(ID = TECHNICAL2)
ContentModel
ETD
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