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Assessing the in vitro bioavailability of tangeretin and its derivatives in Caco-2 cell model
Descriptive
TitleInfo
Title
Assessing the in vitro bioavailability of tangeretin and its derivatives in Caco-2 cell model
Name
(type = personal)
NamePart
(type = family)
Nian
NamePart
(type = given)
Si
DisplayForm
Si Nian
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
HUANG
NamePart
(type = given)
QINGRONG
DisplayForm
QINGRONG HUANG
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
HO
NamePart
(type = given)
CHI-TANG
DisplayForm
CHI-TANG HO
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Wu
NamePart
(type = given)
Qingli
DisplayForm
Qingli Wu
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(encoding = w3cdtf);
(qualifier = exact)
2015
DateOther
(qualifier = exact);
(type = degree)
2015-01
CopyrightDate
(encoding = w3cdtf);
(qualifier = exact)
2015
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Flavonoids are widely distributed in fruits, vegetables and grains. Tangeretin (TAN) is one of the most abundant polymethoxyflavones (PMFs), which is found almost exclusively in citrus peel. In present work, we applied silica gel column chromatography to purify tangeretin from bitter orange citrus peel extracts mixture. Despite of the wide bioactivities documented, tangeretin has relatively low bioavailability due to its low water solubility, which is a major limiting their application as functional neutraceuticals. Therefore, hydroxylation and acetylation reaction was carried out to improve the solubility of TAN. Moreover, the bioactivity and bioavailability of TAN, 5-OH-TAN and 5-Ac-TAN were evaluated using cell viability assay and transport assay on Caco-2 human colon carcinoma cell line. When compared to TAN, stronger anti-proliferation activities were stronger in 5-OH-TAN and 5-Ac-TAN among which 5-Ac-TAN showed the strongest inhibition. The Caco-2 monolayer transport assays were in good agreement that 5-Ac-TAN had better ability to across through cell membranes. Interestingly, 5-OH-TAN and 5-Ac-TAN were subjected to cellular transformation when passing through the cellular membrane. While 5-OH-TAN was partially methylated to TAN, 5-Ac-TAN was transformed into both TAN and 5-OH-TAN, which in total was transported in higher concentration from apical compartment to basolateral side than 5-OH-TAN group. In summery, 5-Ac-TAN had the strongest bioactivity of inhibiting the growth of Caco-2 colon carcinoma cells than TAN and 5-OH-TAN. In addition, 5-Ac-TAN also higher membrane permeability than 5-OH-TAN in Caco-2 monolayer cells. Therefore, functional group on C5 position seems to have critical effect on the compounds’ biological abilities as well as the bioavailability.
Subject
(authority = RUETD)
Topic
Food Science
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_6184
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (x, 59 p. : ill.)
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
Flavonoids--Physiological effect
Subject
(authority = ETD-LCSH)
Topic
Citrus
Subject
(authority = ETD-LCSH)
Topic
Cancer cells
Note
(type = statement of responsibility)
by Si Nian
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3PN97BN
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Nian
GivenName
Si
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2015-01-16 15:20:38
AssociatedEntity
Name
Si Nian
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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