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Mesenchymal stromal cells in alginate dressings to enhance chronic wound healing
Descriptive
TitleInfo
Title
Mesenchymal stromal cells in alginate dressings to enhance chronic wound healing
Name
(type = personal)
NamePart
(type = family)
Faulknor
NamePart
(type = given)
Renea Alexandrine
NamePart
(type = date)
1988-
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Renea Alexandrine Faulknor
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Berthiaume
NamePart
(type = given)
Francois
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Francois Berthiaume
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Maguire
NamePart
(type = given)
Tim
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Tim Maguire
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Schloss
NamePart
(type = given)
Rene
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Rene Schloss
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Banerjee
NamePart
(type = given)
Debrabata
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Debrabata Banerjee
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
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2015
DateOther
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2015-05
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2015
Place
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(type = code)
xx
Language
LanguageTerm
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(type = code)
eng
Abstract
(type = abstract)
Chronic wounds are “stuck” in an inflammatory state characterized by hypoxia and high levels of inflammatory mediators such as inflammatory proteins, white blood cells and bacteria. The hostile environment of chronic wounds readily breaks down growth factors needed to promote wound healing. Recent studies have shown that mesenchymal stem/stromal cells (MSCs), which are non-hematopoietic stem cells, possess “anti-inflammatory” properties. These cells secrete factors that mitigate inflammation and promote wound healing of normal wounds. However, a major limitation with using MSCs is their ability to migrate away from the wound site. Therefore, we have generated a novel bioactive bandage made with alginate to immobilize the MSCs at the wound site. The goal of this project, unlike growth factor depots, is to have the MSCs provide sustained release of soluble factors to the wound and potentially modulate their secretion pattern depending on the wound environment. We hypothesize that MSCs can enhance wound healing by decreasing inflammation in the wound and increasing wound contraction to close the wound. This occurs through their secretion of different soluble factors lacking in chronic wounds and through communication with wound healing cells such as macrophages and fibroblasts. Our results show that the alginate microenvironment does not alter MSC viability or secretion. Immobilized MSCs increased wound closure rate in a diabetic mouse model. We observed that this enhancement could in part be due to the MSCs’ ability to promote differentiation of macrophages to the anti-inflammatory “M2” phenotype and differentiation of fibroblasts into α-smooth muscle actin myofibroblasts that deposit collagen and mediate wound contraction to close the wounds. In human chronic wounds, hypoxia may impair these responses; however, we found that MSCs were able to promote differentiation even in presence of hypoxic conditions.
Subject
(authority = RUETD)
Topic
Biomedical Engineering
Subject
(authority = ETD-LCSH)
Topic
Mesenchymal stem cells
Subject
(authority = ETD-LCSH)
Topic
Wounds and injuries
Subject
(authority = ETD-LCSH)
Topic
Wound healing
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_6353
PhysicalDescription
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electronic resource
InternetMediaType
application/pdf
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text/xml
Extent
1 online resource (xi, 91 p. : ill.)
Note
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Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Renea Alexandrine Faulknor
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3BC41CV
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Faulknor
GivenName
Renea
MiddleName
Alexandrine
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2015-04-14 10:05:52
AssociatedEntity
Name
Renea Faulknor
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(point = start)
2015-05-31
DateTime
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2015-11-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after November 30th, 2015.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
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windows xp
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