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Myristoylated CIL-7 is required for polycystin associated behaviors and extracellular vesicle biogenesis in C. elegans
Descriptive
TitleInfo
Title
Myristoylated CIL-7 is required for polycystin associated behaviors and extracellular vesicle biogenesis in C. elegans
Name
(type = personal)
NamePart
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Maguire
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(type = given)
Julie Elizabeth
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Julie Elizabeth Maguire
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(authority = RULIB)
author
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Barr
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Dr. Maureen M.
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Dr. Maureen M. Barr
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Advisory Committee
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chair
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Driscoll
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Dr. Monica
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Dr. Monica Driscoll
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Advisory Committee
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(authority = RULIB)
internal member
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(type = personal)
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(type = family)
Rongo
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(type = given)
Dr. Christopher
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Dr. Christopher Rongo
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Advisory Committee
Role
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internal member
Name
(type = personal)
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(type = family)
Grant
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(type = given)
Dr. Barth
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Dr. Barth Grant
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Advisory Committee
Role
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outside member
Name
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NamePart
Rutgers University
Role
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(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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school
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Text
Genre
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theses
OriginInfo
DateCreated
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2015
DateOther
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(type = degree)
2015-05
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2015
Place
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xx
Language
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(type = code)
eng
Abstract
(type = abstract)
PKD-2 and LOV-1 are a putative mechanosensory transient receptor potential (TRP) channel-receptor polycystin complex that is required for C. elegans male mating behaviors. PKD-2 and LOV-1 localize to cilia found at the end of dendrites of male-specific sensory neurons, which include the CEMs in the head and the RnB neurons and the HOB neuron in the tail. pkd-2 and lov-1 mutants are defective in responding to hermaphrodites and locating the hermaphrodite vulva (Barr, DeModena et al. 2001). Mutations in PKD1 and PKD2, the human homologs of lov-1 and pkd-2, cause autosomal dominant polycystic kidney disease (ADPKD). As in C. elegans, the mammalian TRP polycystins localize to cilia on renal epithelial cells. The proper placement of PKD-2 to cilia involves several known factors, including LOV-1, intraflagellar transport, and the kinesin-3 KLP-6 (Bae, Qin et al. 2006, Peden, Barr 2005, Hu, Wittekind et al. 2007, Morsci, Barr 2011). The focus of my research revolved around two mutants, cil-7(my16) and cil-1(my15) that were identified through a forward genetic screen for PKD-2::GFP ciliary localization [Cil] defective mutants (Bae, Lyman-Gingerich et al. 2008, Bae, Qin et al. 2006). The emphasis of my research work was on the cil-7(my16) mutant. Loss of CIL-7 function resulted in a defect in the release of PKD-2::GFP containing extracellular vesicles (EVs) into the external environment, and transmission electron microscopy (TEM) showed that EVs remain trapped in the cephalic lumen. I showed that the myristoylation modification of CIL-7 is required for CIL-7 protein function in PKD-2 localization and C. elegans male mating behavior. CIL-7 myristoylation increases CIL-7 association as EV cargo. These studies firmly establish C. elegans as an ideal model in which to investigate EV cargo, such as CIL-7, and the acylation modifications that are necessary to become EV-associated.
Subject
(authority = RUETD)
Topic
Neuroscience
Subject
(authority = ETD-LCSH)
Topic
Caenorhabditis elegans
Subject
(authority = ETD-LCSH)
Topic
Cilia and ciliary motion
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
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ETD_6245
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (vii, 74 p. : ill.)
Note
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Ph.D.
Note
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Includes bibliographical references
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by Julie Elizabeth Maguire
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Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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NjNbRU
Identifier
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doi:10.7282/T3CR5W7W
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
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The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Maguire
GivenName
Julie
MiddleName
Elizabeth
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2015-04-02 13:34:42
AssociatedEntity
Name
Julie Maguire
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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2015-05-31
DateTime
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2017-05-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 30th, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ETD
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windows xp
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