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Hypoglycemic effects of stilbene glycoside from Polygonum multiflorum in type 2 diabetes and its mechanism of action

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TitleInfo
Title
Hypoglycemic effects of stilbene glycoside from Polygonum multiflorum in type 2 diabetes and its mechanism of action
Name (type = personal)
NamePart (type = family)
Tang
NamePart (type = given)
Wenping
DisplayForm
Wenping Tang
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
HO
NamePart (type = given)
CHI-TANG
DisplayForm
CHI-TANG HO
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Diabetes is one of the leading causes of death in the U. S. The number of people with diabetes has been increasing dramatically, and is expected to reach 366 million worldwide by 2030. Type 2 diabetes, affecting more than 90% of diabetes patients, is strongly associated with oxidative stress and is characterized by insulin resistance. There has been a strong need for safe and effective hypoglycemic agents due to side effects from anti-diabetic drugs. Polygonum multiflorum (PM) has long been used as a tonic in traditional Chinese medicine. The medicinal effects of PM are believed to be mediated through its strong antioxidant activity. Our objective is to test if PM extract has anti-diabetic effect, and to identify its active compounds. We gave 0.075% PM extract to KK CgAy/J type 2 diabetic mice in drinking water and after 7 weeks, PM-treated mice had significantly lower glucose level than control mice (p<0.003). We explored possible mechanisms with Elisa and Western Blotting and suggested that the effect was through maintaining β cell function. The major peak in the extract, also the major active compound in PM, trans-2,3,5,4’-tetrahydroxystilbene 2-O-β-glucopyranoside (trans-SG) was evaluated using the same animal model, however, no hypoglycemic effect was found. In exploring efficacious compounds, we identified cis-SG in PM extract and induced isomerization from trans- to cis- SG with UV light. In a high fat-induced type 2 diabetic mice model, crude extract containing cis- and trans- SG with the ratio of 2:3 exhibited significant hypoglycemic capacity, while pure trans-SG and crude extract with the ratio of 1:20 did not. The mechanism of differential anti-diabetic activities of trans- and cis- SG are investigated using PEPCK assay with HepG2 liver cell culture, and both isomers could effectively suppress Dex/cAMP induced PEPCK transcription, with cis-SG offering slightly better results. We also utilized high fat-induced CF-1 male mice to study the anti-diabetic effect of these two isomers. Both SGs were effective in lowering blood glucose in this model, and cis-SG improved glucose intolerance while trans-SG did not. And from the HOMA model the effect in the animal study was found to be through ameliorating insulin resistance.
Subject (authority = RUETD)
Topic
Food Science
Subject (authority = ETD-LCSH)
Topic
Diabetes--Treatment
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6694
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (viii, 115 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Wenping Tang
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3BG2R0W
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Tang
GivenName
Wenping
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-09-01 15:56:20
AssociatedEntity
Name
Wenping Tang
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2017-10-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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