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The effect of substrate stiffness on uropathogenic E. coli infection
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TitleInfo
Title
The effect of substrate stiffness on uropathogenic E. coli infection
Name (type = personal)
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Keklak
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Julia T.
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1991-
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Julia T. Keklak
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author
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Klein
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Eric A
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Eric A Klein
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Advisory Committee
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chair
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Yakoby
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Nir Yakoby
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internal member
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Jongmin
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Jongmin Nam
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Advisory Committee
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Rutgers University
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degree grantor
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Camden Graduate School
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school
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theses
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DateCreated (qualifier = exact)
2016
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2016-05
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2016
Place
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xx
Language
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eng
Abstract (type = abstract)
Both mammalian and bacterial cells respond to environmental cues and then elicit various physiological responses. Mechanical cues, such as surface attachment, have been noted as a critical part of bacterial infection through interaction with mechanosensors and the actin cytoskeleton. Urinary tract infections are a sufficient model for studying attachment due to the mechanism of bacterial infection. The cytoskeleton mediates various cell processes that are involved with infection, such as endocytosis and vesicle trafficking. The disruption of the host actin cytoskeleton leads to increased uropathogenic E. coli infection (UPEC). Since substrate stiffness mediates changes in host cell physiology through cytoskeletal interaction, we explored the UPEC infection process on polyacrylamide gels of differing stiffnesses. We found that UPEC escapes endocytic vesicles and replicates within the cytoplasm on soft gels, yet are trapped within lysosomal-associated membrane protein (LAMP-1) vesicles on stiff surfaces. Cytoplasmic replication is consistent with previous in vivo studies. Increased infection was also found on soft gels when compared with stiff substrates. These findings may provide a more accurate cell culturing model for future UPEC infection studies.
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Topic
Biology
Subject (authority = ETD-LCSH)
Topic
Escherichia coli infections
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Rutgers University Electronic Theses and Dissertations
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ETD_7355
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electronic resource
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application/pdf
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text/xml
Note
Supplementary File: Julia Keklak Copyright Images
Extent
1 online resource (iii, 38 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
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by Julia T. Keklak
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Camden Graduate School Electronic Theses and Dissertations
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rucore10005600001
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Identifier (type = doi)
doi:10.7282/T3DJ5HR9
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

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The author owns the copyright to this work.
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Name
FamilyName
Keklak
GivenName
Julia
MiddleName
T.
Role
Copyright Holder
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Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2016-05-03 10:24:13
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Name
Julia Keklak
Role
Copyright holder
Affiliation
Rutgers University. Camden Graduate School
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Type
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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