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Nanotechnology approach for targeted treatment of triple negative breast cancer
Descriptive
TitleInfo
Title
Nanotechnology approach for targeted treatment of triple negative breast cancer
Name
(type = personal)
NamePart
(type = family)
Al-Mahmood
NamePart
(type = given)
Sumayah Abed Ahmed
NamePart
(type = date)
1982-
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Sumayah Abed Ahmed Al-Mahmood
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(authority = RULIB)
author
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(type = personal)
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(type = family)
Minko
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(type = given)
Tamara
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Tamara Minko
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Advisory Committee
Role
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(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Michniak-Kohn
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(type = given)
Bozena
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Bozena Michniak-Kohn
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Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
You
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(type = given)
Guofeng
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Guofeng You
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
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NamePart
Rutgers University
Role
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(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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school
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Text
Genre
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theses
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2016
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2016-05
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2016
Place
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xx
Language
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eng
Abstract
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Breast cancer is one of the most devastating diseases worldwide. Triple negative breast cancer cells (TNBCs) are defined by the lack of progesterone receptor (PR), estrogen receptor (ER), and epidermal growth factor receptor 2 (EGFR2) expressions. TNBCs account for 10%- 20% of all breast carcinomas. The study is aimed at examining the efficacy of gefitinib and EGFR-targeted siRNA delivered by liposomes for treating triple negative breast cancer (TNBC). The experiments were carried out using two types of human breast cancer (BC) cell lines MCF-7 (estrogen positive BC, EPBC) and MDA-MB 231(TNBC). EGFR-targeted siRNA and gefitinib were delivered by cationic and neutral liposomes, respectively. A fluorescence microscope was used to study cellular internalization of labeled liposomes and siRNA. The expression of the targeted mRNA was performed using quantitative reverse transcription PCR. Finally, cytotoxicity of liposomal siRNA and gefitinib alone or in combination was measured using the modified MTT assay with appropriate controls. It was found that liposomes effectively delivered siRNA into both types of BC cells and suppressed the expression of targeted EGFR mRNA. However, formulations without gefitinib did not influence significantly on the viability of BC cells. Free drug demonstrated the ability to kill both types of cancer cells. Nevertheless, toxicity of gefitinib in TNBC was 2.5 times lower when compared with EPBC cells. The delivery of the drug by liposomes significantly enhanced its toxicity (1.2 and 2.5 times in EPBC and TNBC, respectively). The combination of liposomal siRNA and liposomal gefitinib demonstrated exceptionally high cytotoxicity when compared with the free drug (143 and 62 times higher in EPBC and TNBC, respectively). Suppression of EGFR mRNA effectively suppressed resistance of TNBC cells to gefitinib. The data obtained support the proposed approach and showed high potential of liposomal EGFR siRNA in combination with liposomal gefitinib in treatment of TNBC.
Subject
(authority = RUETD)
Topic
Pharmaceutical Science
Subject
(authority = ETD-LCSH)
Topic
Nanotechnology
Subject
(authority = ETD-LCSH)
Topic
Breast--Cancer--Treatment
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
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ETD_7302
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (xi, 82 p. : ill.)
Note
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M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Sumayah Abed Ahmed Al-Mahmood
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3RB76RC
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
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The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Al-Mahmood
GivenName
Sumayah
MiddleName
Abed Ahmed
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2016-04-20 14:23:02
AssociatedEntity
Name
Sumayah Al-Mahmood
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
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2016-05-31
DateTime
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2017-05-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ETD
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windows xp
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1.4
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2016-04-20T14:09:18
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2016-04-20T14:09:18
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