RUcore: Rutgers University Community Repository
Investigation of heat shock on tendon and ligament cells in vitro
Descriptive
TitleInfo
Title
Investigation of heat shock on tendon and ligament cells in vitro
Name
(type = personal)
NamePart
(type = family)
Shah
NamePart
(type = given)
Jay V.
NamePart
(type = date)
1993-
DisplayForm
Jay V. Shah
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Freeman
NamePart
(type = given)
Joseph W
DisplayForm
Joseph W Freeman
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Dunn
NamePart
(type = given)
Michael G
DisplayForm
Michael G Dunn
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Olabisi
NamePart
(type = given)
Ronke M
DisplayForm
Ronke M Olabisi
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2016
DateOther
(qualifier = exact);
(type = degree)
2016-10
CopyrightDate
(encoding = w3cdtf);
(qualifier = exact)
2016
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Tendons and ligaments (T/L) are collagenous connective tissues that play a role in locomotion and mechanical stabilization of the joints. These tissues possess relatively low cellularity and vascularity, resulting in a long and potentially incomplete healing response following injury. For sub-failure injuries such as strains and sprains, the most common course of treatment is an implementation of rest, ice, compression, and elevation. However, this method relies mostly on the tissue's natural ability to heal, which can leave the tissue prone to re-injury and failure. As a potential aid in the T/L healing process, we investigated the effects of thermal stress on human tenocytes in vitro. Heat shock has been demonstrated to improve regeneration in other tissues of the musculoskeletal system, including bone and muscle. Exposure of cells to an increase in temperature induces the production of heat shock proteins (HSPs) which act as molecular chaperones to assist in protein assembly and transport. In this project, heat shock at 40°, 44°, and 48°C was applied for 5, 10, 15, or 20 minutes. The data shows an increase in cellular proliferation following 15 and 20 minutes of thermal conditioning at 44° and 48°C as soon as 4 hours after heat shock. Protein secretion and gene expression of collagens type I and III and TGF-β suggest that that the heat shock response of tenocytes is similar to that of natural wound healing. Lastly, an in vitro scratch wound healing assay revealed that the use of heat may hasten the recovery time following T/L subfailure injuries.
Subject
(authority = RUETD)
Topic
Biomedical Engineering
Subject
(authority = ETD-LCSH)
Topic
Tendons
Subject
(authority = ETD-LCSH)
Topic
Ligaments
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_7685
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (viii, 45 p. : ill.)
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Jay V. Shah
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3B56N3D
Genre
(authority = ExL-Esploro)
ETD graduate
Back to the top
Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Shah
GivenName
Jay
MiddleName
V.
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2016-09-29 15:41:10
AssociatedEntity
Name
Jay Shah
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2016-10-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2017-05-02
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 2nd, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
Back to the top
Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
CreatingApplication
Version
1.6
DateCreated
(point = end);
(encoding = w3cdtf);
(qualifier = exact)
2016-09-22T22:34:43
DateCreated
(point = end);
(encoding = w3cdtf);
(qualifier = exact)
2016-09-29T15:27:08
ApplicationName
Microsoft® Office Word 2007
Back to the top
Statistical Profile