Naringenin is an important natural product of the flavonoid family and possesses various biological activities. It has a complex chemical structure and is mainly produced by plants. However, naringenin production by either plant extraction or chemical synthesis is challenging, due to low productivity and yield associated with these methods. Microbial co-culture engineering has been found to be a robust approach for production of a wide range of biochemical products and holds strong potential for efficient complex natural product biosynthesis. In this project, novel E.coli-E.coli co-cultures were constructed to produce naringenin from simple carbon substrates, such as glucose and glycerol. The cultivation conditions for the E.coli- E.coli co-culture approach was systematically studied for bioproduction optimization. It was found that the E.coli-E.coli co-culture approach showed superior production performance compared to the mono culture approach. The findings of this project demonstrate the potential of engineering microbial co-cultures for heterologous biosynthesis of complex molecules.
Subject (authority = RUETD)
Topic
Chemical and Biochemical Engineering
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_8293
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (vii, 40 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Subject (authority = ETD-LCSH)
Topic
Escherichia coli
Note (type = statement of responsibility)
by Vijaydev Ganesan
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.