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53BP1 regulates dna double-strand break repair in a mouse “BRCA-like” model by a non-resection mechanism
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Misenko, Sarah Marie. 53BP1 regulates dna double-strand break repair in a mouse “BRCA-like” model by a non-resection mechanism. Retrieved from https://doi.org/doi:10.7282/T3GF0XNM

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Title53BP1 regulates dna double-strand break repair in a mouse “BRCA-like” model by a non-resection mechanism
NameMisenko, Sarah Marie (author); Bunting, Samuel F (chair); Steward, Ruth (internal member); Jin, Victor (internal member); Denzin, Lisa (outside member); Rutgers University; School of Graduate Studies
Date Created2017
Other Date2017-10 (degree)
SubjectBiochemistry, DNA damage, DNA repair
Extent1 online resource (x, 118 p. : ill.)
DescriptionDNA end resection is believed to be a step that influences the choice between the two major DNA double-strand break (DSB) repair pathways: homologous recombination (HR) and non-homologous end joining (NHEJ). Deficiencies in either of these pathways can increase predisposition to cancer, a common example being BRCA1 deficient breast and ovarian cancer. Specifically, cells deficient in HR have been considered to be “BRCA-like”. BLM/Dna2 and Exo1 serve as the two main pathways for long-range resection in yeast, but the significance of long-range resection for genome maintenance has not been shown in a primary mammalian model. We found that BlmΔ/ΔExo1-/- cells show synergistic genomic instability compared to single knockout controls, reduced resection, and decreased HR. BlmΔ/ΔExo1-/- cells also show a defect in the G2M checkpoint, attributed to a lack of resected DNA to maintain the checkpoint activation. This suggests that co-deletion of BLM and Exo1 abolishes long-range resection resulting in a “BRCA-like” model. Deleting 53BP1, a protein believed to block resection, restores genomic integrity and HR in BlmΔ/ΔExo1-/- cells, but resection is not restored. This data suggests a role for 53BP1 in restoring HR that is independent of its role in inhibiting DNA end resection.
NotePh.D.
NoteIncludes bibliographical references
Noteby Sarah Marie Misenko
Genretheses, ETD doctoral
Persistent URLhttps://doi.org/doi:10.7282/T3GF0XNM
Languageeng
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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