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The role of heterochromatin in RNAi-mediated transcription repression in C. elegans
Descriptive
TitleInfo
Title
The role of heterochromatin in RNAi-mediated transcription repression in C. elegans
Name
(type = personal)
NamePart
(type = family)
Kalinava
NamePart
(type = given)
Natallia
NamePart
(type = date)
1980-
DisplayForm
Natallia Kalinava
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Gu
NamePart
(type = given)
Sam Guoping
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Sam Guoping Gu
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Driscoll
NamePart
(type = given)
Monica
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Monica Driscoll
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Gartenberg
NamePart
(type = given)
Marc
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Marc Gartenberg
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Hampsey
NamePart
(type = given)
Michael
DisplayForm
Michael Hampsey
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
School of Graduate Studies
Role
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(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2018
DateOther
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(type = degree)
2018-01
CopyrightDate
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(qualifier = exact)
2018
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
The germline nuclear RNAi pathway in C. elegans can lead to histone H3 lysine 9 trimethylation (H3K9me3) and transcriptional silencing at target genes. H3K9me3 heterochromatin induced by either exogenous double-stranded RNA (dsRNA) or endogenous siRNA (endo-siRNA) is highly specific to the target loci and is trans-generationally heritable. Despite these features, the role of H3K9me3 in siRNA-mediated establishment and maintenance of transcriptional silencing and its inheritance in C. elegans is unclear. It is also not known which of H3K9me3 histone methyltransferases (HMT) function in an RNAi-dependent manner. In this study, we took combined genetic, biochemical, CRISPR-Cas9 genome editing, cell biology and computational approaches to address these questions. Here we demonstrate that siRNA-mediated H3K9me3 requires combined activities of three H3K9 histone methyltransferases (HMTs): MET-2, SET-25, and SET-32. Surprisingly, loss of high-magnitude of H3K9me3 in set-32; met-2 set-25 mutant worms has no effect on the transcriptional silencing at the native nuclear RNAi targets. In addition, the exogenous dsRNA-induced transcriptional silencing and heritable RNAi at oma-1, a well-established nuclear RNAi reporter gene, are completely resistant to the loss of H3K9me3. Repair of germline nuclear Argonaut HRDE-1 fully restore silencing of nuclear RNAi targets. However, loss of SET-32 showed trans-generational delay in re-establishment of transcriptional silencing. We propose that H3K9me3 is dispensable for the maintenance of siRNA-mediated transcriptional silencing. However, H3K9me HMT SET-32 promotes the onset of nuclear RNAi-mediated transcriptional silencing.
Subject
(authority = RUETD)
Topic
Cell and Developmental Biology
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_8527
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (x, 108 p. : ill.)
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
RNA interference
Note
(type = statement of responsibility)
by Natallia Kalinava
RelatedItem
(type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier
(type = local)
rucore10001600001
Location
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(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T30K2CSK
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Kalinava
GivenName
Natallia
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2017-10-09 13:09:48
AssociatedEntity
Name
Natallia Kalinava
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
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windows xp
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1.3
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Mac OS X 10.11.6 Quartz PDFContext
DateCreated
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2017-11-10T00:12:54
DateCreated
(point = end);
(encoding = w3cdtf);
(qualifier = exact)
2017-11-10T00:12:54
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