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Measuring and modeling the influence of relative humidity, temperature, and buffer type on the survival and growth of Enterobacter aerogenes
Descriptive
TitleInfo
Title
Measuring and modeling the influence of relative humidity, temperature, and buffer type on the survival and growth of Enterobacter aerogenes
Name
(type = personal)
NamePart
(type = family)
Igo
NamePart
(type = given)
Matthew
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Matthew Igo
Role
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(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Schaffner
NamePart
(type = given)
Donald
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Donald Schaffner
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Matthews
NamePart
(type = given)
Karl
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Karl Matthews
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Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
SALVI
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(type = given)
DEEPTI
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DEEPTI SALVI
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
School of Graduate Studies
Role
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school
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Text
Genre
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theses
OriginInfo
DateCreated
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2018
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2018-05
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2018
Place
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xx
Language
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(type = code)
eng
Abstract
(type = abstract)
Survival of bacteria on surfaces is an important part in understanding cross-contamination of food. The effects of temperature and relative humidity appear to play important roles in understanding how bacteria survive on surfaces. Surface type and inoculum matrix also appear to influence bacterial survival. This study examines how relative humidity, temperature, and inoculum matrix effected the survival of non-pathogenic Enterobacter aerogenes on common surfaces stainless steel, PVC, and ceramic tile. While surface type seemed to have little effect on survival, temperature showed a clear effect. E. aerogenes survived better at 7°C at 15 and 50% relative humidity on all surfaces. Inoculum matrix composition influenced survival and even allowed growth under some high RH conditions. Cells suspended in distilled water experienced a larger decrease in concentration immediately after inoculation on the surface vs. 0.1% peptone or 1% PBS. Cells suspended in 1% PBS showed a sharper decline in survival after 120 hours compared to 0.1% peptone both 15 and 50% relative humidity but cells in both matrices had similar tailing up to 3 weeks. Cells suspended in 0.1% peptone showed greatest growth and had the highest population density (~8 log CFU/ml) when the organism was inoculated into 10 mL of peptone. Cells suspended in PBS or distilled water showed ~2 log CFU/ml increase in concentration. When cells in 0.1% peptone were inoculated onto a stainless steel coupon and placed at 100% RH, concentration increased to ~7 log CFU/coupon after a lag time of ~24 hours while cells in 1% PBS increased to ~5 log CFU/coupon followed by a decline over 3 weeks. DMFit and GinaFit software could model inactivation on surfaces at all conditions other than 100% RH at 21°C.
Subject
(authority = RUETD)
Topic
Food Science
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_8764
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (ix, 45 p. : ill.)
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
Enterobacter aerogenes
Note
(type = statement of responsibility)
by Matthew Igo
RelatedItem
(type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier
(type = local)
rucore10001600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T38S4TBF
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Igo
GivenName
Matthew
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2018-04-06 10:46:39
AssociatedEntity
Name
Matthew Igo
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
(point = start)
2018-05-31
DateTime
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(qualifier = exact);
(point = end)
2018-11-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after November 30th, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ETD
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windows xp
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1.3
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2018-04-06T15:51:49
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2018-04-06T15:51:49
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