DescriptionStress is a principal factor in promoting smoking lapse and relapse to cigarette smoking in women. Cognitive reappraisal of stress (CR) and heart rate variability biofeedback (HRVb) have both demonstrated positive effects on the stress response and related outcomes, but have never been explored together for their effects on smoking-related outcomes. Sixty-eight adult women, who smoke 10+ cigarettes daily, abstained from nicotine and tobacco for 12 hours prior to a lab visit in which they completed questionnaires and were randomized to practice an intervention of CR and HRVb, or neutral control tasks. All participants then completed three stressful tasks, including a Stroop task to measure cognitive performance. Heart rate variability (HRV) data were collected throughout the study visit, in addition to smoking craving and negative affect assessments at baseline, post-intervention, post-stress, and post-recovery. Results showed a significant difference in craving decrease between experimental conditions, with the Intervention condition showing a significant decrease in smoking craving from baseline after 10 minutes of HRVb. There were no significant differences between conditions in Stroop task performance or negative affect throughout the study. HRV results revealed group differences in heart rate increase during stress, and decrease after stress, with the Intervention group exhibiting greater volatility in heart rate. Overall, findings of this study indicate that practicing HRVb for 10 minutes results in a significant decrease in smoking craving in female daily smokers with 12 hours of abstinence, and multiple practice sessions of CR and HRVb may be needed to protect against the effect of stress on craving, negative affect, cognitive performance, or HRV. Future research will need to replicate these findings in a larger sample and explore the efficacy of practicing HRVb and CR together over multiple practice sessions in smoking cessation treatment.