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Metabolic regulation underlying stalk synthesis in Caulobacter crescentus
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de Young, Kevin. Metabolic regulation underlying stalk synthesis in Caulobacter crescentus. Retrieved from https://doi.org/doi:10.7282/t3-ngdz-nx12

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TitleMetabolic regulation underlying stalk synthesis in Caulobacter crescentus
Namede Young, Kevin (author); Klein, Eric (chair); Rutgers University; Camden Graduate School
Date Created2019
Other Date2019-05 (degree)
SubjectBiology, Bacteria -- Morphology
Extent1 online resource (vii, 59 pages) : illustrations
DescriptionCaulobacter crescentus is a gram-negative α-proteobacterium known for its characteristic adhesive stalk and a tolerance to nutrient deprivation. Found mostly in oligotrophic (nutrient-depleted) aquatic environments, Caulobacter responds to changes in resource availability with dynamic metabolic and morphological alterations. We isolated a mutant strain with reduced stalk length; the mutation mapped to gene CC3617, a mannose 6-phosphate isomerase. During phosphate starvation, wild-type cells maintain a 1:1 ratio of mannose 6-phosphate (M6P) and fructose 6-phosphate (F6P). Our CC3617 mutant strain, by contrast, has a relative increase in F6P. The decrease in M6P correlates with low levels of exopolysaccharide and O-antigen synthesis. Interestingly, we find that the CC3617 mutant strain does not readily enter stationary phase; rather it continuously increases cell number and represses stationary-phase gene expression. Forced induction of stationary phase promotes stalk length recovery in the CC3617 mutant strain. Furthermore, deletion of stationary phase genes in wild-type cells produce a reduction in stalk length.
NoteM.S.
NoteIncludes bibliographical references
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/t3-ngdz-nx12
LanguageEnglish
CollectionCamden Graduate School Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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