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Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates

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TitleInfo
Title
Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates
Name (type = personal)
NamePart (type = family)
Hogquist
NamePart (type = given)
Stephen Joseph
NamePart (type = date)
1995-
DisplayForm
Stephen Joseph Hogquist Jr.
Role
RoleTerm (authority = RULIB)
author
NamePart (type = termsOfAddress)
Jr.
Name (type = personal)
NamePart (type = family)
Zahn
NamePart (type = given)
Jeffrey D
DisplayForm
Jeffrey D Zahn
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Shreiber
NamePart (type = given)
David
DisplayForm
David Shreiber
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Labazzo
NamePart (type = given)
Kristen
DisplayForm
Kristen Labazzo
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
School of Graduate Studies
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (keyDate = yes); (qualifier = exact)
2020
DateOther (encoding = w3cdtf); (qualifier = exact); (type = degree)
2020-01
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Abstract (type = abstract)
Electroporation is the technique of applying an electric field to a cell to achieve temporary membrane permeabilization and allow the passage of drugs, chemicals, or DNA through the compromised cell membrane. Electroporation technology is presently an area of research interest due to its versatility and potential benefit to various investigational therapies including vaccines and cell and gene therapies. The transfection efficiency and cell viability following electroporation experiments depend strongly on various parameters such as the applied electric field, cell type used, and the contents and electrical characteristics of the buffer solution through which a field is applied. This study aims to demonstrate a technique by which a lower voltage can be used to electroporate cells with the goal of increasing cell viability and permeabilization efficiency with plasmid DNA and propidium iodide when compared to existing techniques. This is achieved utilizing 3T3 cells attached to a biocompatible, 60µm thick nanoporous alumina membrane substrate with 100nm pores. Computational modeling predicts that applying an electric field through this nanoporous substrate in a conductive buffer solution results in electric field amplification near nanopores to achieve electroporation of cells adherent to the substrate, while maintaining a lower field strength elsewhere in the remaining area surrounding a cell. As many existing electroporation experiments work with cell suspensions in cuvettes to achieve electroporation, this technique presents a method to achieve electroporation of adherent cells. Results suggest that delivery cargo of interest can be electrophoretically driven through these nanopores and enter a cell with an electroporation pulse under select electric field conditions. Here, a technique is described to further investigate the application of nanoporous substrates in electroporation experiments. Furthermore, the impact of electric field strength amplification through alumina nanopores is demonstrated by the successful permeabilization of cell membranes with delivery markers.
Subject (authority = RUETD)
Topic
Biomedical Engineering
Subject (authority = LCSH)
Topic
Electroporation
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_10555
PhysicalDescription
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application/pdf
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text/xml
Extent
1 online resource (x, 79 pages) : illustrations
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/t3-dcrw-4b86
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Hogquist Jr.
GivenName
Stephen
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2020-01-13 15:17:02
AssociatedEntity
Name
Stephen Hogquist Jr.
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

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ETD
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windows xp
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1.7
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DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2020-01-13T13:20:38
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2020-01-13T13:20:38
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