Kellogg, Jack Ericson. Metacommunity species delimitation and population genetics in tropical tank bromeliad phytotelmata. Retrieved from https://doi.org/doi:10.7282/t3-w1k7-h933
DescriptionStudying whole communities and ecosystems is seldom performed which makesempirical studies in community and ecosystem ecology difficult to execute. An ecosystem that is often utilized though is the individual ecosystems contained in tank bromeliad phytotelmata that are comprised of mostly larval invertebrates and insects. The inhabitants of these systems are drastically understudied making morphological species identifications very difficult or impossible which inhibits studies pertaining to biodiversity. DNA barcoding and a single-locus maximum likelihood tree-based species delimitation method were used for the first time in this system to estimate patterns of diversity and gene flow in a naturally occurring experimental setup on samples collected along an elevation gradient in the Monteverde region of Costa Rica. This naturally occurring experimental setup contains three different habitat types (cloud forest, wet primary rainforest, and dry primary rainforest). Biodiversity is expected to change along the elevation gradient as temperature, as well as levels of gene flow because of particular dispersal barriers that may exist in the changing landscape. Operational barcode units were successfully delimited and results suggest the presence of the mid domain effect with further investigation required. Species found along the entirety of the elevation gradient were targeted to assess gene flow among populations. Certain species shared genetic information along the entire gradient, and some species showed a level of population divergence indicating a dispersal barrier or perhaps cryptic speciation. Both of which require a more robust dataset to answer those questions.