RUcore: Rutgers University Community Repository
The effects of different forms of vitamin A on intestinal health: insights from a genetic mouse model of vitamin A deficiency
Descriptive
TitleInfo
Title
The effects of different forms of vitamin A on intestinal health: insights from a genetic mouse model of vitamin A deficiency
Name
(type = personal)
NamePart
(type = family)
Honarbakhsh
NamePart
(type = given)
Maryam
NamePart
(type = date)
1980-
DisplayForm
Maryam Honarbakhsh
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Quadro
NamePart
(type = given)
Loredana
DisplayForm
Loredana Quadro
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Breslin
NamePart
(type = given)
Paul A.S.
DisplayForm
Paul A.S. Breslin
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Roopchand
NamePart
(type = given)
Diana
DisplayForm
Diana Roopchand
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Vetriani
NamePart
(type = given)
Costantino
DisplayForm
Costantino Vetriani
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Blaner
NamePart
(type = given)
William S.
DisplayForm
William S. Blaner
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
School of Graduate Studies
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
Genre
(authority = ExL-Esploro)
ETD doctoral
OriginInfo
DateCreated
(qualifier = exact);
(encoding = w3cdtf);
(keyDate = yes)
2020
DateOther
(type = degree);
(qualifier = exact);
(encoding = w3cdtf)
2020-10
CopyrightDate
(encoding = w3cdtf);
(qualifier = exact)
2020
Language
LanguageTerm
(authority = ISO 639-3:2007);
(type = text)
English
Abstract
(type = abstract)
Vitamin A deficiency (VAD) is one of the most widespread micronutrient deficiencies affecting hundreds of millions of children and pregnant women in over half of the countries worldwide, despite the implementation of numerous intervention programs. Emerging evidence suggests that VAD can impact intestinal microbiota diversity and fitness suggesting a combined effect of dietary vitamin A and status. Not only vitamin A can be obtained from food of animal origin as preformed vitamin A, but also from vegetables and fruits containing provitamin A carotenoids, mainly as βC. In fact, βC is the most abundant dietary source of vitamin A worldwide, and often the sole source of the vitamin in certain regions of the world. Yet, its impact on intestinal functions and microbiome is unknown, especially during VAD.
The goal of this study was to understand the impact of different forms of dietary vitamin A (preformed retinoids or provitamin A carotenoids) vs. vitamin A status on intestinal health during VAD. To address this question, we used a genetic mouse model of VAD, the Lrat-/-Rbp-/- mice. Unable to store and deliver retinoids (vitamin A and its derivatives) to peripheral tissues, these mice display a tenuous vitamin A status. Fecal 16S rRNA gene analysis showed that VAD in our genetic model was concomitant with fecal microbial dysbiosis and dietary vitamin A did not impact the fecal microbial taxonomic profile. Also, VAD resulted in impaired structure and functions of the intestinal barrier as manifested by reduced mucins and antimicrobial defense, leaky gut, increased inflammation and oxidative stress, and impaired mucosal immunocytokine profiles. We also showed that that -carotene, as the sole source of the vitamin, not only improved the vitamin A status of the vitamin A deficient mutant mice but also improved intestinal dysfunctions and modified the taxonomic profile of the fecal microbiome of VAD.
The goal of this study was to understand the impact of different forms of dietary vitamin A (preformed retinoids or provitamin A carotenoids) vs. vitamin A status on intestinal health during VAD. To address this question, we used a genetic mouse model of VAD, the Lrat-/-Rbp-/- mice. Unable to store and deliver retinoids (vitamin A and its derivatives) to peripheral tissues, these mice display a tenuous vitamin A status. Fecal 16S rRNA gene analysis showed that VAD in our genetic model was concomitant with fecal microbial dysbiosis and dietary vitamin A did not impact the fecal microbial taxonomic profile. Also, VAD resulted in impaired structure and functions of the intestinal barrier as manifested by reduced mucins and antimicrobial defense, leaky gut, increased inflammation and oxidative stress, and impaired mucosal immunocytokine profiles. We also showed that that -carotene, as the sole source of the vitamin, not only improved the vitamin A status of the vitamin A deficient mutant mice but also improved intestinal dysfunctions and modified the taxonomic profile of the fecal microbiome of VAD.
Subject
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject
(authority = LCSH)
Topic
Vitamin A deficiency
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_11095
PhysicalDescription
Form
(authority = gmd)
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xvii, 230 pages) : illustrations
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
RelatedItem
(type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier
(type = local)
rucore10001600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/t3-8ajh-a231
Back to the top
Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
honarbakhsh
GivenName
maryam
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2020-08-21 16:40:57
AssociatedEntity
Name
maryam honarbakhsh
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
Type
Embargo
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2020-10-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2022-10-31
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2022.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
Back to the top
Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
CreatingApplication
Version
1.7
ApplicationName
Microsoft® Word for Microsoft 365
DateCreated
(point = end);
(encoding = w3cdtf);
(qualifier = exact)
2020-08-25T10:52:52
DateCreated
(point = end);
(encoding = w3cdtf);
(qualifier = exact)
2020-08-25T10:52:52
Back to the top
Statistical Profile