Kaur, Amanpreet. Investigation of the role of EPFL4,5,6 genes in stomata formation under osmotic stress. Retrieved from https://doi.org/doi:10.7282/t3-5681-z127
DescriptionStomata are micropores on the epidermis of the aerial parts of plants that facilitate gas exchange for photosynthesis and transpiration. Stomatal development is plastic to the environment, and is tightly regulated by various internal and external signals. A group of secretory ligands in the EPIDERMAL PATTERNING FACTOR (EPF) family are involved in stomatal development, among which several are responsive to the environment. EPF ligands are perceived by a group of receptor kinases named the ERECTA family, and a co- receptor TOO MANY MOUTHS (TMM) as a modulator. Our preliminary data reveal that stomatal development is inhibited by osmotic stress. In addition, EPFL5 and EPFL6 are induced by the osmotic treatment. In this study, by analyzing the stomatal phenotype of higher-ordered epf mutants using genetic and cell biology approaches, I found that the stomatal inhibition phenotype can be partially recovered by the removal of EPFL4, EPFL5, or EPFL6. Additionally, our results suggested that EPFL5 or EPFL6 contributes more than EPFL4 in the stomatal inhibition under osmotic stress. To summarize, our study indicates that EPFL5 and EPFL6 are two negative regulators of stomatal formation under osmotic stress.