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Studies of viral tethering and its effect in the early phase of MLV life cycle
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Addaquay, Araba. Studies of viral tethering and its effect in the early phase of MLV life cycle. Retrieved from https://doi.org/doi:10.7282/T30R9RF1

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TitleStudies of viral tethering and its effect in the early phase of MLV life cycle
NameAddaquay, Araba (author); Roth, Monica (chair); Grant, Barth (internal member); ZARATEIGUI, MIKEL (internal member); Rutgers University; Graduate School - New Brunswick
Date Created2016
Other Date2016-01 (degree)
SubjectCell and Developmental Biology, Mouse leukemia viruses, Retroviruses
Extent1 online resource (v, 30 p. : ill.)
DescriptionThe p12 proteins designed to encode peptide sequences from viral tethering proteins was shown to rescue murine leukemia virus (MLV) p12 PM14 lethal mutants (Schneider et al., 2013). However, the viral tethering protein from Kaposi’s sarcoma associated herpes virus (KSHV) latency-associated nuclear antigen encoding a nuclear localization signal, LANA(1-32), could not rescue MLV p12 PM14. This isolate p12 with LANA(1-32) fused to GFP was found to bind tightly to mitotic chromosomes. We show that virus bearing p12 LANA(1-32) PM14 retain the group-specific antigen (Gag) precursor inside the nucleus and that insertion of a nuclear export signal (NES) is able to counterbalance the effect of the NLS (nuclear localization signal). However, viral kinetics of MLV p12 LANA(1-32) PM14 with or without the NES indicates that the virus is still not viable. This suggests that the original hypothesis of the tight tethering domain that is p12 LANA(1-32) is detrimental to MLV infection.
NoteM.S.
NoteIncludes bibliographical references
Noteby Araba Addaquay
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T30R9RF1
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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