Anti-inflammatory effect of resveratrol metabolite, δ-viniferin, on lipopolysaccharide-stimulated murine macrophage
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Hsieh, Pei-Hsuan.
Anti-inflammatory effect of resveratrol metabolite, δ-viniferin, on lipopolysaccharide-stimulated murine macrophage. Retrieved from
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TitleAnti-inflammatory effect of resveratrol metabolite, δ-viniferin, on lipopolysaccharide-stimulated murine macrophage
Date Created2016
Other Date2016-01 (degree)
Extent1 online resource (x, 56 p. : ill.)
DescriptionInflammation is a complicated physiological and pathological process in response to stimuli. In general, a controlled inflammatory response is a beneficial defense system, which provides protection against infection. However, it can become detrimental when the response is prolonged and dysregulated called chronic inflammation. Over the past several years, chronic inflammation has been recognized to be the root of various human diseases, including neurological disorders, metabolic disorders, obesity, cardiovascular diseases and cancers. Resveratrol is a well-known natural antioxidative and anti-inflammatory compound from grapes. In the previous study, the antioxidative properties of resveratrol metabolites have been suggested to be more active than resveratrol. δ-Viniferin is a main resveratrol dehydrodimer metabolite and has been identified in grape cell cultures and wines. It possesses two resorcinol skeletons, which are determinants of radical scavenging and antioxidative potential. Hence, we researched in the anti-inflammatory effect of δ-viniferin. We prepared this compound in vitro by the oxidative dimerization of resveratrol with horseradish peroxidase in the presence of H2O2. In this study, the cytotoxic and anti-inflammatory effects on LPS-stimulated murine macrophage were examined. According to the data, we found that δ-viniferin suppressed the levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and phosphorylated inhibitor κBα (p-IκBα) protein expressions, as well as the down-stream product, nitric oxide, in murine RAW 264.7 cells induced with lipopolysaccharide (LPS). Moreover, the inhibitory effects of δ-viniferin on inflammation associated signaling pathways, such as the blockade of LPS-induced IκBα phosphorylation and degradation, the decrease of PI3K and Akt phosphorylation, and the reduction of LPS-induced transcriptional activity of NF-κB. According to the results, δ-viniferin might have potential to be developed into an effective anti-inflammatory agent.
NoteM.S.
NoteIncludes bibliographical references
Noteby Pei-Hsuan Hsieh
Genretheses, ETD graduate
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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