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Screening and characterization of a mutant library of the microalga nannochloropsis oceanica for growth and lipid production at high CO2 conditions
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Vu, Hoa. Screening and characterization of a mutant library of the microalga nannochloropsis oceanica for growth and lipid production at high CO2 conditions. Retrieved from https://doi.org/doi:10.7282/T3474F13

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TitleScreening and characterization of a mutant library of the microalga nannochloropsis oceanica for growth and lipid production at high CO2 conditions
NameVu, Hoa (author); Dismukes, Charles (chair); Bhattacharya, Debashish (internal member); Lun, Desmond (internal member); Rutgers University; School of Graduate Studies
Date Created2017
Other Date2017-10 (degree)
SubjectMicrobial Biology, Microalgae, Lipids
Extent1 online resource (vii, 34 p. : ill.)
DescriptionNannochloropsis is a genus of microalgae that produces substantial amounts of storage lipids referred to as triacylglycerides that are derived from fatty acids. These products are precursors to important dietary lipids for use in oils and as replacements for hydrocarbon fuels. To enhance lipid production in Nannochloropsis oceanica CCMP 1779 we applied an insertional mutagenesis approach to produce a library of 1200 strains containing single insertions located randomly in the genome. The collection was screened for growth at high CO2 (≤10%) and low pH, and for high lipid content. Seven mutants were selected for quantitative assays and characterization of photosynthetic efficiency. All 7 strains and the wild type (WT) grow fastest as measured by growth rate and final dry biomass at 2% CO2. The WT had a higher growth rate and biomass production compared with the mutant strains at 2% and 10% CO2. There was pH and CO2-dependence of the growth rate of mutants and the WT. A level of 10% CO2 was a stress condition for Nannocholoropsis oceanica CCMP 1779. One strain (G2) grown at 2% and 10% CO2 had a higher lipid content by Nile Red fluorescence than the WT. When quantified by lipid extraction, this strain did not show a higher lipid content than the WT by day 15 of growth.
NoteM.S.
NoteIncludes bibliographical references
Noteby Hoa Vu
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T3474F13
Languageeng
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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