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Process of creating a more efficient real-time quantitative PCR assay to detect Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, and Borrelia miyamotoi that inhabit deer ticks
Descriptive
TitleInfo
Title
Process of creating a more efficient real-time quantitative PCR assay to detect Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, and Borrelia miyamotoi that inhabit deer ticks
Name
(type = personal)
NamePart
(type = family)
Allan
NamePart
(type = given)
Rachael
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Rachael Allan
Role
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(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Toledo
NamePart
(type = given)
Alvaro
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Alvaro Toledo
Affiliation
Advisory Committee
Role
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(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Zylstra
NamePart
(type = given)
Gerben
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Gerben Zylstra
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Kerkhof
NamePart
(type = given)
Lee
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Lee Kerkhof
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
School of Graduate Studies
Role
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school
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Text
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theses
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2019
DateOther
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(type = degree)
2019-05
Language
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(authority = ISO 639-3:2007);
(type = text)
English
Abstract
(type = abstract)
Ixodes scapularis, the most abundant tick species in New Jersey, is a vector for a host of human diseases, including Lyme disease, Anaplasmosis, Babesiosis, and B. miyamotoi. The prevalence of these diseases continues to steadily increase, and it has become critical to develop molecular methods to detect them. This project focuses on transitioning from a standard PCR and gel electrophoresis methodology to real-time PCR to screen for four common pathogens found in deer ticks – B. burgdorferi, B. miyamotoi, A. phagocytophilum, and B. microti. Primers and probes were created to target genes that were unique to each pathogen. They were tested using standard PCR and gel electrophoresis and were then optimized with real-time PCR using SYBR Green and TaqMan methodologies. The primer specificity was also assessed using tick DNA as a template utilizing a SYBR Green methodology. 158 deer ticks were tested for the presence of pathogens – 11.39% of deer ticks tested positive for B. burgdorferi, 6.33% tested positive for B. microti, 6.96% tested positive for B. miyamotoi, and 10.76% tested positive for A. phagocytophilum. With all of these steps completed, a multiplex PCR assay can be created to screen for all four pathogens in one single test tube.
Subject
(authority = RUETD)
Topic
Microbial Biology
Subject
(authority = LCSH)
Topic
Tick-borne diseases -- Testing
Subject
(authority = LCSH)
Topic
Anaplasmosis -- Testing
Subject
(authority = LCSH)
Topic
Babesiosis -- Testing
Subject
(authority = LCSH)
Topic
Borrelia burgdorferi -- Testing
Subject
(authority = LCSH)
Topic
Borrelia -- Testing
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_9656
PhysicalDescription
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application/pdf
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text/xml
Extent
1 online resource (xviii, 108 pages) : illustrations
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
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TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier
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rucore10001600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/t3-t6j6-fn98
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Allan
GivenName
Rachael
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2019-04-01 22:11:01
AssociatedEntity
Name
Rachael Allan
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
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windows xp
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1.5
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2019-04-02T01:42:02
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2019-04-02T01:42:03
ApplicationName
www.ilovepdf.com
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