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Effect of delayed gene therapy treatment in spinal cord injury
Descriptive
TitleInfo
Title
Effect of delayed gene therapy treatment in spinal cord injury
Name
(type = personal)
NamePart
(type = family)
Castro-Pedrido
NamePart
(type = given)
Sofia
NamePart
(type = date)
1995
DisplayForm
Sofia Castro-Pedrido
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(type = text)
author
Name
(type = personal)
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(type = family)
Cai
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(type = given)
Li
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Li Cai
Affiliation
Advisory Committee
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(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Vazquez
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(type = given)
Maribel
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Maribel Vazquez
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Advisory Committee
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Berthiaume
NamePart
(type = given)
Francois
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Francois Berthiaume
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
School of Graduate Studies
Role
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school
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Text
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theses
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ETD graduate
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2020
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2020-10
Language
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(authority = ISO 639-3:2007);
(type = text)
English
Abstract
Gene therapies are being developed which seek to repair the spinal cord and give patients back their mobility by lentivirus-mediated expression of neurogenic transcription factors. Through targeted overexpression of key transcription factors, endogenous cells can be motivated to remodel the site of injury. Previously, our lab has identified a gene therapy in a mouse model of hemisection spinal cord injury (SCI) that, when injected immediately after injury, produces locomotor functional recovery by decreasing cell death, promoting proliferation, and activating neural stem cells. However, in clinical settings, therapeutic intervention would happen hours or even days after the injury. Thus, we aim to determine the effect of a delayed treatment for effective gene therapies of spinal cord injuries. Our hypothesis is that injecting the therapeutic agent some time after injury will still be effective to promote functional recovery. In this study, we used a lateral hemisection SCI mouse model and injected the lentiviral gene therapy one day after injury. The spinal cord tissue was harvested, stained, and analyzed at three different timepoints after the injection to determine the effects of the therapy on the site of injury. Our results showed successful overexpression of the target transcription factor and consequently, decreased cell death in the subacute phase of injury, increased neural stem cell activation, and an increase in cholinergic neurons around the injury site. Our results show that the therapy successfully remodeled the site of injury and could potentially lead to functional recovery in a long-term study. Improving the SCI mouse model for testing gene therapies will better predict their efficacy in clinical settings.
Subject
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Topic
Delayed treatment
Subject
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Topic
Biomedical Engineering
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Rutgers University Electronic Theses and Dissertations
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ETD_11154
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application/pdf
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text/xml
Extent
1 online resource (vi, 28 pages)
Note
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M.S.
Note
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Includes bibliographical references
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School of Graduate Studies Electronic Theses and Dissertations
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rucore10001600001
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NjNbRU
Identifier
(type = doi)
doi:10.7282/t3-q57s-cv91
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Rights
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The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Castro-Pedrido
GivenName
Sofia
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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2020-09-21 12:22:37
AssociatedEntity
Name
Sofia Castro-Pedrido
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
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Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Embargo
DateTime
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2020-10-31
DateTime
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2022-10-31
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2022.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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2020-09-22T20:19:05
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