Home
Resource
3D printed spectrophotometer for drug monitoring in tuberculosis patients for global health applications
PDF
PDF format is widely accepted and good for printing.
Plug-in required
PDF-1(1.37 MB)
Citation & Export
View Usage Statistics
Staff View

Citation & Export
Hide

Simple citation

Hooda, Pragya. 3D printed spectrophotometer for drug monitoring in tuberculosis patients for global health applications. Retrieved from https://doi.org/doi:10.7282/t3-5csd-6714

Export

Click here for information about Citation Management Tools at Rutgers.

Statistics
Hide

Description

Title3D printed spectrophotometer for drug monitoring in tuberculosis patients for global health applications
NameHooda, Pragya (author); Hassan, Umer (chair); Pierce, Mark (internal member); Shrirao, Anil (internal member); Rutgers University; School of Graduate Studies
Date Created2021
Other Date2021-01 (degree)
SubjectTuberculosis, Biomedical Engineering
Extent1 online resource (vi, 37 pages) : illustrations
DescriptionSignificant progress has been made to fight Tuberculosis (TB), and while many effective diagnoses and treatments have saved millions of lives, the infectious disease still remains to be one of the leading causes of death today. An infected patient can be prescribed with antibiotic drugs such as Rifampin for a certain period of time until treatment is complete. Drug resistant strains of TB have started to grow, creating an urgency to control the spread of this disease. Rapid diagnostic tools and different delivery methods have reduced the mortality rate from TB, however, there is still an urgent need to develop a point-of-care (POC) test that can improve the treatment process for TB patients. Here, we aim to create a POC device that can detect the amount of antibiotic in a biological sample (e.g. urine). We developed and tested a portable three-dimensional (3D) printed spectrophotometer for different biological assays and compared the results with a laboratory standard table-top instrument spectrophotometer. First, we demonstrated the accuracy of the 3D printed spectrophotometer using a Bradford Assay and found a good correlation (R2 = 0.96) between our 3D printed and instrument spectrophotometer. Subsequently, assay is modified to process smaller sample volume using microcuvettes in the setup. We found a correlation coefficient of R2 = 0.92 between our 3D printed and instrument spectrophotometer. The device was then tested for a Rifampin assay and a standard curve is developed in both spiked PBS and urine samples. The correlation results from the rifampin assay will shine light on future diagnostic and drug monitoring testing for TB patients. In conclusion, our results show that our 3D printed spectrophotometer has a strong correlation compared to the real instrument spectrophotometer for absorbance-based measurements. In future, POC instruments such as our 3D printed spectrophotometer can be used by the medical staff for active TB management, and possibly to test patients response to new emerging strains of TB. Proactive diagnosis and treatment of TB patients can significantly reduce the death rate, and in turn improve the quality of human life in global health settings.
NoteM.S.
NoteIncludes bibliographical references
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/t3-5csd-6714
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
Version 8.5.5
Rutgers University Libraries - Copyright ©2024