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The necessity of tRNA-Val-CAC-1-1 derived tRNA fragments (tRFs) in drosophila
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Gattone, Nicholas. The necessity of tRNA-Val-CAC-1-1 derived tRNA fragments (tRFs) in drosophila. Retrieved from https://doi.org/doi:10.7282/t3-v322-ax10

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TitleThe necessity of tRNA-Val-CAC-1-1 derived tRNA fragments (tRFs) in drosophila
NameGattone, Nicholas (author); Yakoby, Nir (chair); Rutgers University; Camden Graduate School
Date Created2021
Other Date2021-01 (degree)
SubjectBiology, RNA
Extent1 online resource (vii, 36 pages)
DescriptionSmall non-coding RNAs, such as micro-RNAs (miRNAs) or small interfering RNAs (siRNAs) are critical regulators of gene expression during tissue development. tRNA-derived fragments are a newly discovered class of small non-coding RNA that participate in different biological processes, some of which are attributed to post-transcriptional regulation similar to miRNAs. Using a computational pipeline designed to find tRF targets by mirroring a miRNA-like seed-based targeting mechanism, we identified that the tRNA-Val-CAC-1-1 contains a seed sequence that may target epidermal growth factor receptor (EGFR) inhibitor Sprouty. Using CRISPR/Cas9 genome engineering, this tRNA was deleted. The transgenic fly remained heterozygous, suggesting a necessary role for fly survival. The RNA-seq data from the heterozygous fly did demonstrate small transcriptional changes for target genes in both the ovary and embryo. In addition, a global change in the distribution of small RNAs was found, suggesting a possible stress reaction on the small RNA level from the targeted deletion. Further CRISPR experiments are underway to determine the reason for the unviability of the tRNA-Val-CAC-1-1 homozygous mutant.
NoteM.S.
NoteIncludes bibliographical references
Genretheses, External ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/t3-v322-ax10
LanguageEnglish
CollectionCamden Graduate School Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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