DescriptionHair cells are mechanoreceptors of the inner ear that convert sounds into electrical signals to be perceived by the brain. The gradual loss of hair cells is common after a lifetime of chronic exposure to loud noises. Mammalian hair cells lack the ability to regenerate after embryonic development. Advancements in regenerative medicine strategies have been employed to restore function to damaged organs. The use of stem cells can regenerate and replace damaged hair cells. Understanding how stem cells differentiate into hair cells will provide insight into regeneration. To study hair cell regeneration, we employed an inner ear organoid system. Immortalized multipotent otic progenitor (iMOP) cells that differentiate into spiral ganglion neurons, supporting cells, and hair cells were used to generate organoids. This study tests whether a bioreactor facilitates sensory epithelial differentiation. The bioreactor agitates the medium to promote nutrient diffusion in the cultures. After ten days of differentiation, iMOP-derived organoids were collected, and relative changes in transcripts and protein markers that correspond to neurons, supporting cells, and hair cells were determined. The use of a bioreactor increased the percentage of cells expressing MYO6, a hair cell marker. The percentage of TUBB3 labeled neurons also increased while GFAP labeled supporting cells remained the same. Relative levels of Myo6 mRNA did not increase in any of the culture conditions while relative levels of Tubb3 mRNA significantly increased in all cultures compared to control samples.