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A fluorescence spectroscopy and fluorescence anisotropy investigation of the heteroaggregation of alginate microparticles and chitosan nanoparticles
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Pathak, Chinmay. A fluorescence spectroscopy and fluorescence anisotropy investigation of the heteroaggregation of alginate microparticles and chitosan nanoparticles. Retrieved from https://doi.org/doi:10.7282/T38S4RR0

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TitleA fluorescence spectroscopy and fluorescence anisotropy investigation of the heteroaggregation of alginate microparticles and chitosan nanoparticles
NamePathak, Chinmay (author); Shapley, Nina (chair); Chiew, Yee (internal member); Dutt, Meenakshi (internal member); Rutgers University; Graduate School - New Brunswick
Date Created2015
Other Date2015-05 (degree)
SubjectChemical and Biochemical Engineering, Chitosan, Nanoparticles
Extent1 online resource (xv, 112 p. : ill.)
DescriptionHeteroaggregation is the aggregation in a mixed particle system where particles differ in size, chemical composition and charge. This heteroaggregation phenomenon has great importance in the natural environment as well as in industrial processes. The heteroaggregates have a wide range of applications such as particle stabilization, drug delivery and encapsulation. Previous studies of heteroaggregates have involved rigid spherical particles. In this work, we have used hydrophilic hydrogel particles which are oppositely charged and soft. These hydrogel particles, in particular, chitosan and alginate, have gained interest due to their biodegradability and nontoxicity as well as multiple delivery mechanisms. This study focuses on understanding the heteroaggregation behavior of chitosan nanoparticles and alginate microparticles and several factors affecting their interaction. Fluorescence spectroscopy was used to characterize and identify boundaries between several heteroaggregate morphologies, or “phases,” in the system of chitosan nanoparticles and alginate microparticles. Optical microscopy was used to observe the aggregate phases formed by fluorescently tagged chitosan nanoparticles mixed with alginate microparticles. The effect of the concentration ratio of alginate microparticles and chitosan nanoparticles was also explored. Next, the interaction between these particles was studied for varying pH of the system as pH is an important factor where micro- and nanoparticles are used as a drug delivery system. The interaction behavior was examined regarding the changes in solution ionic strength. Furthermore, fluorescence anisotropy, which is correlated to mobility, was used to observe aggregates’ formation with varying concentration and pH. Finally, fluorescence spectroscopy was performed in situ in a vial containing an alginate-chitosan system.
NoteM.S.
NoteIncludes bibliographical references
Noteby Chinmay Pathak
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T38S4RR0
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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